PDBsum entry 3oq7

Hydrolase

PDB id: 3oq7

Contents

Protein chain

99 a.a.

Waters ×114

PDB id:

3oq7

Name:

Hydrolase

Title:

Crystal structures of multidrug-resistant clinical isolate 769 HIV-1 protease variants

Structure:

HIV-1 protease. Chain: a. Fragment: unp residues 1-99. Engineered: yes. Other_details: multidrug-resistant clinical isolate # 769 HIV-1 protease

Source:

Human immunodeficiency virus 1. HIV-1. Organism_taxid: 11676. Strain: mdr769. Gene: pol. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.71Å R-factor: 0.196 R-free: 0.223

Authors:

R.S.Yedidi,G.Proteasa,J.L.Martinez-Cajas,J.F.Vickrey,P.D.Martin, Z.Wawrzak,L.C.Kovari

Key ref:

R.S.Yedidi et al. (2011). Contribution of the 80s loop of HIV-1 protease to the multidrug-resistance mechanism: crystallographic study of MDR769 HIV-1 protease variants. Acta Crystallogr D Biol Crystallogr, 67, 524-532. PubMed id: 21636892

Date:

02-Sep-10 Release date: 06-Apr-11

Protein chain

P35963  (POL_HV1Y2) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate YU-2)

Seq: 1435 a.a.

Struc: 99 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.- - ?????
• Enzyme class 2: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: E.C.3.1.-.-
• Enzyme class 5: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Acta Crystallogr D Biol Crystallogr 67:524-532 (2011)

PubMed id: 21636892

Contribution of the 80s loop of HIV-1 protease to the multidrug-resistance mechanism: crystallographic study of MDR769 HIV-1 protease variants.

R.S.Yedidi, G.Proteasa, J.L.Martinez, J.F.Vickrey, P.D.Martin, Z.Wawrzak, Z.Liu, I.A.Kovari, L.C.Kovari.

ABSTRACT

No abstract given.