PDBsum entry 3olh

Transferase

PDB id: 3olh

Contents

Protein chain

278 a.a. *

Ligands

SO4 ×2

Metals

_NA

Waters ×75

* Residue conservation analysis

PDB id:

3olh

Name:

Transferase

Title:

Human 3-mercaptopyruvate sulfurtransferase

Structure:

3-mercaptopyruvate sulfurtransferase. Chain: a. Fragment: unp residuse 11-289. Synonym: mst. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mpst, tst2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.50Å R-factor: 0.226 R-free: 0.270

Authors:

T.Karlberg,R.Collins,C.H.Arrowsmith,H.Berglund,C.Bountra,A.M.Edwards, S.Flodin,A.Flores,S.Graslund,M.Hammarstrom,I.Johansson,T.Kotenyova, E.Kouznetsova,M.Moche,P.Nordlund,T.Nyman,C.Persson,P.Schutz,A.Sehic, M.I.Siponen,A.G.Thorsell,L.Tresaugues,S.Van Den Berg,E.Wahlberg, J.Weigelt,M.Welin,H.Schuler,Structural Genomics Consortium (Sgc)

Key ref:

T.Karlberg et al. Human 3-Mercaptopyruvate sulfurtransferase. To be published, .

Date:

26-Aug-10 Release date: 29-Sep-10

Protein chain

P25325  (THTM_HUMAN) -  3-mercaptopyruvate sulfurtransferase from Homo sapiens

Seq: 297 a.a.

Struc: 278 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.8.1.2 - 3-mercaptopyruvate sulfurtransferase.
• Reaction: 2-oxo-3-sulfanylpropanoate + [thioredoxin]-dithiol = [thioredoxin]- disulfide + hydrogen sulfide + pyruvate + H⁺
• Cofactor: Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site