PDBsum entry 3oii

Ribosomal protein

PDB id: 3oii

Contents

Protein chains

216 a.a. *

Ligands

SAH ×2

GOL

Waters ×224

* Residue conservation analysis

PDB id:

3oii

Name:

Ribosomal protein

Title:

Crystal structure of saccharomyces cerevisiae nep1/emg1 bound to s- adenosylhomocysteine

Structure:

Essential for mitotic growth 1. Chain: a, b. Synonym: nucleolar essential protein 1. Engineered: yes

Source:

Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Gene: emg1, nep1, ylr186w, l9470.5. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.85Å R-factor: 0.174 R-free: 0.216

Authors:

S.R.Thomas,A.Szyk,N.Laronde-Leblanc

Key ref:

S.R.Thomas et al. (2011). Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis. Nucleic Acids Res, 39, 2445-2457. PubMed id: 21087996

Date:

19-Aug-10 Release date: 01-Dec-10

Protein chains

Q06287  (NEP1_YEAST) -  Ribosomal RNA small subunit methyltransferase NEP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 252 a.a.

Struc: 216 a.a.

Enzyme reactions

• Enzyme class: E.C.2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1.
• Reaction: pseudouridine₁₁₉₁ in yeast 18S rRNA + S-adenosyl-L-methionine = N₁- methylpseudouridine₁₁₉₁ in yeast 18S rRNA + S-adenosyl-L-homocysteine + H⁺

pseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = N(1)- methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-homocysteine + H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Nucleic Acids Res 39:2445-2457 (2011)

PubMed id: 21087996

Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis.

S.R.Thomas, C.A.Keller, A.Szyk, J.R.Cannon, N.A.Laronde-Leblanc.

ABSTRACT

No abstract given.