PDBsum entry 3ogj

Transferase

PDB id: 3ogj

Contents

Protein chains

128 a.a.

Ligands

CMP ×2

PO4 ×2

Waters ×5

PDB id:

3ogj

Name:

Transferase

Title:

Crystal structure of partial apo (92-227) of cgmp-dependent protein kinase

Structure:

Prkg1 protein. Chain: a, b, c, d. Fragment: cyclic nucleotie binding domain. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.75Å R-factor: 0.183 R-free: 0.251

Authors:

J.J.Kim,G.Huang,T.K.Kwon,P.Zwart,J.Headd,C.Kim

Key ref:

J.J.Kim et al. (2011). Co-crystal structures of PKG Iβ (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding. Plos One, 6, e18413. PubMed id: 21526164

Date:

16-Aug-10 Release date: 11-May-11

Protein chains

Q13976  (KGP1_HUMAN) -  cGMP-dependent protein kinase 1 from Homo sapiens

Seq: 671 a.a.

Struc: 128 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.12 - cGMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = CMP) matches with 81.48% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = CMP) matches with 81.48% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos One 6:e18413 (2011)

PubMed id: 21526164

Co-crystal structures of PKG Iβ (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding.

J.J.Kim, D.E.Casteel, G.Huang, T.H.Kwon, R.K.Ren, P.Zwart, J.J.Headd, N.G.Brown, D.C.Chow, T.Palzkill, C.Kim.

ABSTRACT

No abstract given.