PDBsum entry 3o3f

Hydrolase/DNA-RNA hybrid

PDB id

3o3f

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Contents

			Protein chain

					222 a.a. *

			DNA/RNA

			Metals

					_MG ×2

			Waters ×146

* Residue conservation analysis

PDB id:

3o3f

Links

Name:

Hydrolase/DNA-RNA hybrid

Title:

T. Maritima rnase h2 d107n in complex with nucleic acid substrate and magnesium ions

Structure:

Ribonuclease hii. Chain: a. Synonym: rnase hii. Engineered: yes. Mutation: yes. DNA (5'-d( Gp Ap Ap Tp Cp Ap Gp Gp Tp Gp Tp C)-3'). Chain: c. Engineered: yes. DNA/RNA (5'-d( Gp Ap Cp Ap C)-r(p C)-d(p Tp Gp Ap Tp Tp C)-

Source:

Thermotoga maritima. Organism_taxid: 2336. Gene: rnhb, tm_0915. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

2.00Å

R-factor:

0.194

R-free:

0.244

Authors:

M.P.Rychlik,H.Chon,S.M.Cerritelli,P.Klimek,R.J.Crouch,M.Nowotny

Key ref:

M.P.Rychlik et al. (2010). Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage. Mol Cell, 40, 658-670. PubMed id: 21095591

Date:

24-Jul-10

Release date:

08-Dec-10

PROCHECK

	Headers

	References

Protein chain

Q9X017 (RNH2_THEMA) - Ribonuclease HII from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

Seq: Struc:					238 a.a. 222 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

		G-A-A-T-C-A-G-G-T-G-T-C 12 bases
		G-A-C-A-C-C-T-G-A-T-T-C 12 bases



		Enzyme reactions

Enzyme class:

E.C.3.1.26.4 - ribonuclease H.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Endonucleolytic cleavage to 5'-phosphomonoester.

	Mol Cell 40:658-670 (2010)
PubMed id: 21095591

Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage.
M.P.Rychlik, H.Chon, S.M.Cerritelli, P.Klimek, R.J.Crouch, M.Nowotny.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21177858

M.Figiel, H.Chon, S.M.Cerritelli, M.Cybulska, R.J.Crouch, and M.Nowotny (2011).
The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects.

J Biol Chem, 286, 10540-10550.

PDB code:

3puf

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.