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PDBsum entry 3o2s
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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3o2s

 

 

 

 

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Contents
Protein chains
311 a.a. *
190 a.a. *
Ligands
PO4
Waters ×76
* Residue conservation analysis
PDB id:
3o2s
Name: Hydrolase
Title: Crystal structure of the human symplekin-ssu72 complex
Structure: Symplekin. Chain: a. Fragment: n-terminal domain. Engineered: yes. RNA polymerase ii subunit a c-terminal domain phosphatase ssu72. Chain: b. Synonym: ctd phosphatase ssu72. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: sympk, spk. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ssu72, hspc182, pnas-120. Expression_system_taxid: 562
Resolution:
2.50Å     R-factor:   0.214     R-free:   0.266
Authors: L.Tong,K.Xiang
Key ref: K.Xiang et al. (2010). Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex. Nature, 467, 729-733. PubMed id: 20861839
Date:
22-Jul-10     Release date:   06-Oct-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q92797  (SYMPK_HUMAN) -  Symplekin from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1274 a.a.
311 a.a.
Protein chain
Pfam   ArchSchema ?
Q9NP77  (SSU72_HUMAN) -  RNA polymerase II subunit A C-terminal domain phosphatase SSU72 from Homo sapiens
Seq:
Struc: 		194 a.a.
190 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 2: Chain A: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
   Enzyme class 3: Chain B: E.C.3.1.3.16  - protein-serine/threonine phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate
O-phospho-L-seryl-[protein]
 + H2O
 = L-seryl-[protein]
 +
phosphate
Bound ligand (Het Group name = PO4)
corresponds exactly
O-phospho-L-threonyl-[protein]
 + H2O
 = L-threonyl-[protein]
 +
phosphate
Bound ligand (Het Group name = PO4)
corresponds exactly
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    Added reference    
 
 
Nature 467:729-733 (2010)
PubMed id: 20861839  
 
 
Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex.
K.Xiang, T.Nagaike, S.Xiang, T.Kilic, M.M.Beh, J.L.Manley, L.Tong.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21292162 S.Danckwardt, A.S.Gantzert, S.Macher-Goeppinger, H.C.Probst, M.Gentzel, M.Wilm, H.J.Gröne, P.Schirmacher, M.W.Hentze, and A.E.Kulozik (2011).
p38 MAPK controls prothrombin expression by regulated RNA 3' end processing.
  Mol Cell, 41, 298-310.  
21329879 T.Nagaike, C.Logan, I.Hotta, O.Rozenblatt-Rosen, M.Meyerson, and J.L.Manley (2011).
Transcriptional activators enhance polyadenylation of mRNA precursors.
  Mol Cell, 41, 409-418.  
21204787 Y.Zhang, M.Zhang, and Y.Zhang (2011).
Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue.
  Biochem J, 434, 435-444.
PDB codes: 3omw 3omx
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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