PDBsum entry 3o2q

Hydrolase

PDB id

3o2q

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Contents

			Protein chains

					317 a.a. *


					189 a.a. *

			Ligands

					PRO-THR-SEP-PRO- SER-TYR


					PO4

			Waters ×250

* Residue conservation analysis

PDB id:

3o2q

Links

Name:

Hydrolase

Title:

Crystal structure of the human symplekin-ssu72-ctd phosphopeptide complex

Structure:

Symplekin. Chain: a, d. Fragment: n-terminal domain. Engineered: yes. RNA polymerase ii subunit a c-terminal domain phosphatase ssu72. Chain: b, e. Synonym: ctd phosphatase ssu72. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sympk, spk. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ssu72, hspc182, pnas-120. Synthetic: yes

Resolution:

2.40Å

R-factor:

0.187

R-free:

0.242

Authors:

L.Tong,K.Xiang

Key ref:

K.Xiang et al. (2010). Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex. Nature, 467, 729-733. PubMed id: 20861839

Date:

22-Jul-10

Release date:

06-Oct-10

PROCHECK

	Headers

	References

Protein chains

Q92797 (SYMPK_HUMAN) - Symplekin from Homo sapiens

Seq: Struc:

Seq: Struc:

Seq: Struc:					1274 a.a. 317 a.a.*

Protein chains

Q9NP77 (SSU72_HUMAN) - RNA polymerase II subunit A C-terminal domain phosphatase SSU72 from Homo sapiens

Seq: Struc:					194 a.a. 189 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 6 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

Chains B, E: E.C.3.1.3.16 - protein-serine/threonine phosphatase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
2.	O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

O-phospho-L-seryl-[protein]

H2O

L-seryl-[protein]

phosphate
Bound ligand (Het Group name = SEP)
matches with 50.00% similarity

O-phospho-L-threonyl-[protein]

H2O

L-threonyl-[protein]

phosphate
Bound ligand (Het Group name = SEP)
matches with 50.00% similarity

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Nature 467:729-733 (2010)
PubMed id: 20861839

Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex.
K.Xiang, T.Nagaike, S.Xiang, T.Kilic, M.M.Beh, J.L.Manley, L.Tong.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21292162

S.Danckwardt, A.S.Gantzert, S.Macher-Goeppinger, H.C.Probst, M.Gentzel, M.Wilm, H.J.Gröne, P.Schirmacher, M.W.Hentze, and A.E.Kulozik (2011).
p38 MAPK controls prothrombin expression by regulated RNA 3' end processing.

Mol Cell, 41, 298-310.

21329879

T.Nagaike, C.Logan, I.Hotta, O.Rozenblatt-Rosen, M.Meyerson, and J.L.Manley (2011).
Transcriptional activators enhance polyadenylation of mRNA precursors.

Mol Cell, 41, 409-418.

21204787

Y.Zhang, M.Zhang, and Y.Zhang (2011).
Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue.

Biochem J, 434, 435-444.

PDB codes:

3omw 3omx

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.