PDBsum entry 3nxk

Hydrolase

PDB id: 3nxk

Contents

Protein chains

(+ 1 more) 327 a.a. *

306 a.a. *

Ligands

SO4 ×16

ACY ×3

GOL ×10

Waters ×1060

* Residue conservation analysis

PDB id:

3nxk

Name:

Hydrolase

Title:

Crystal structure of probable cytoplasmic l-asparaginase from campylobacter jejuni

Structure:

Cytoplasmic l-asparaginase. Chain: a, b, c, d, e, f, g, h. Engineered: yes

Source:

Campylobacter jejuni subsp. Jejuni. Organism_taxid: 192222. Strain: nctc 11168. Gene: ansa, cj0029. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.40Å R-factor: 0.169 R-free: 0.228

Authors:

Y.Kim,M.Makowska-Grzyska,N.Maltseva,L.Papazisi,W.F.Anderson, A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

Y.Kim et al. Crystal structure of probable cytoplasmic l-Asparagin from campylobacter jejuni. To be published, .

Date:

14-Jul-10 Release date: 04-Aug-10

Protein chains

Q0PC96  (Q0PC96_CAMJE) -  Cytoplasmic L-asparaginase from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

Seq: 331 a.a.

Struc: 327 a.a.

Protein chain

Q0PC96  (Q0PC96_CAMJE) -  Cytoplasmic L-asparaginase from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

Seq: 331 a.a.

Struc: 306 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G, H: E.C.3.5.1.1 - asparaginase.
• Reaction: L-asparagine + H2O = L-aspartate + NH4⁺

L-asparagine + H2O (Bound ligand (Het Group name = ACY) matches with 44.44% similarity) = L-aspartate + NH4(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site