PDBsum entry 3nrs

Ligase

PDB id: 3nrs

Contents

Protein chain

401 a.a. *

Ligands

TLA ×3

GOL ×3

MES

Waters ×312

* Residue conservation analysis

PDB id:

3nrs

Name:

Ligase

Title:

Crystal structure of ligand-free bifunctional folylpolyglutamate synthase/dihydrofolate synthase from yersinia pestis c092

Structure:

Dihydrofolate:folylpolyglutamate synthetase. Chain: a. Synonym: putative folc bifunctional protein. Engineered: yes

Source:

Yersinia pestis. Organism_taxid: 214092. Strain: co92. Gene: folc, yp_2395, y1602. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.80Å R-factor: 0.174 R-free: 0.192

Authors:

B.Nocek,N.Maltseva,M.Makowska-Grzyska,L.Papazisi,W.Anderson, A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

B.Nocek et al. Crystal structure of ligand-Free bifunctional folylpolyglutamate synthase/dihydrofolate synthase fr yersinia pestis c092. To be published, .

Date:

30-Jun-10 Release date: 04-Aug-10

Protein chain

Q8D0U0  (Q8D0U0_YERPE) -  Dihydrofolate synthase/folylpolyglutamate synthase from Yersinia pestis

Seq: 460 a.a.

Struc: 401 a.a.

Enzyme reactions

• Enzyme class 1: E.C.6.3.2.12 - dihydrofolate synthase.
• Pathway: Folate Biosynthesis (late stages)
• Reaction: 7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H⁺

7,8-dihydropteroate + L-glutamate + ATP (Bound ligand (Het Group name = TLA) matches with 42.86% similarity) = 7,8-dihydrofolate + ADP + phosphate + H(+)

• Enzyme class 2: E.C.6.3.2.17 - tetrahydrofolate synthase.

Pathway:

• Reaction: (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)- 5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H⁺

(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP (Bound ligand (Het Group name = TLA) matches with 42.86% similarity) = (6S)- 5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site