PDBsum entry 3nqs

Oxidoreductase

PDB id: 3nqs

Contents

Protein chains

412 a.a. *

Ligands

HEM ×2

H4B-_NO ×2

AT2 ×2

GOL

EDO ×3

SO4 ×5

BOG

Waters ×598

* Residue conservation analysis

PDB id:

3nqs

Name:

Oxidoreductase

Title:

Crystal structure of inducible nitric oxide synthase with n- nitrosated-pterin

Structure:

Nitric oxide synthase, inducible. Chain: a, b. Fragment: unp residues 66-498. Synonym: inducible no synthase, inducible nos, inos, nos type ii, macrophage nos, mac-nos. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Strain: macrophage. Gene: inosl, nos2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.204 R-free: 0.229

Authors:

R.J.Rosenfeld,E.D.Getzoff,J.A.Tainer

Key ref:

R.J.Rosenfeld et al. (2010). Nitric-oxide synthase forms N-NO-pterin and S-NO-cys: implications for activity, allostery, and regulation. J Biol Chem, 285, 31581-31589. PubMed id: 20659888

Date:

29-Jun-10 Release date: 21-Jul-10

Protein chains

P29477  (NOS2_MOUSE) -  Nitric oxide synthase, inducible from Mus musculus

Seq: 1144 a.a.

Struc: 413 a.a.

Enzyme reactions

• Enzyme class: E.C.1.14.13.39 - nitric-oxide synthase (NADPH).
• Reaction: 2 L-arginine + 3 NADPH + 4 O2 + H⁺ = 2 L-citrulline + 2 nitric oxide + 3 NADP⁺ + 4 H2O

2 × L-arginine + 3 × NADPH + 4 × O2 + H(+) = 2 × L-citrulline + 2 × nitric oxide + 3 × NADP(+) + 4 × H2O (Bound ligand (Het Group name = NO) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 285:31581-31589 (2010)

PubMed id: 20659888

Nitric-oxide synthase forms N-NO-pterin and S-NO-cys: implications for activity, allostery, and regulation.

R.J.Rosenfeld, J.Bonaventura, B.R.Szymczyna, M.J.MacCoss, A.S.Arvai, J.R.Yates, J.A.Tainer, E.D.Getzoff.

ABSTRACT

No abstract given.