PDBsum entry 3npm

Transferase

PDB id: 3npm

Contents

Protein chain

307 a.a.

Ligands

PO4 ×2

Waters ×222

PDB id:

3npm

Name:

Transferase

Title:

Crystal structure of the c47a/a241c disulfide-linked c6 aspartate transcarbamoylase enzyme

Structure:

Aspartate carbamoyltransferase catalytic chain. Chain: a. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k-12. Gene: pyrb, b4245, jw4204. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.178 R-free: 0.205

Authors:

K.R.Mendes,E.R.Kantrowitz

Key ref:

K.R.Mendes and E.R.Kantrowitz (2010). A cooperative Escherichia coli aspartate transcarbamoylase without regulatory subunits . Biochemistry, 49, 7694-7703. PubMed id: 20681545

Date:

28-Jun-10 Release date: 11-May-11

Protein chain

P0A786  (PYRB_ECOLI) -  Aspartate carbamoyltransferase catalytic subunit from Escherichia coli (strain K12)

Seq: 311 a.a.

Struc: 307 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.1.3.2 - aspartate carbamoyltransferase.
• Pathway: Pyrimidine Biosynthesis
• Reaction: carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H⁺

carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+) (Bound ligand (Het Group name = PO4) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 49:7694-7703 (2010)

PubMed id: 20681545

A cooperative Escherichia coli aspartate transcarbamoylase without regulatory subunits .

K.R.Mendes, E.R.Kantrowitz.

ABSTRACT

No abstract given.