PDBsum entry 3nip

Hydrolase

PDB id: 3nip

Contents

Protein chains

(+ 0 more) 316 a.a.

Ligands

16D ×5

Waters ×417

PDB id:

3nip

Name:

Hydrolase

Title:

Crystal structure of pseudomonas aeruginosa guanidinopropionase complexed with 1,6-diaminohexane

Structure:

3-guanidinopropionase. Chain: a, b, c, d, e, f. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: gpua, pa0288. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å R-factor: 0.193 R-free: 0.227

Authors:

S.J.Lee,H.S.Kim,D.J.Kim,H.J.Yoon,K.H.Kim,J.Y.Yoon,J.Y.Jang,H.Im,D.An, S.W.Suh

Key ref:

S.J.Lee et al. (2011). Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol, 175, 329-338. PubMed id: 21600989

Date:

16-Jun-10 Release date: 01-Jun-11

Protein chains

Q9I6K2  (GPUA_PSEAE) -  Guanidinopropionase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 318 a.a.

Struc: 316 a.a.

Enzyme reactions

• Enzyme class: E.C.3.5.3.17 - guanidinopropionase.
• Reaction: 3-guanidinopropanoate + H2O = urea + beta-alanine

3-guanidinopropanoate + H2O = urea (Bound ligand (Het Group name = 16D) matches with 40.00% similarity) + beta-alanine

• Cofactor: Mn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Struct Biol 175:329-338 (2011)

PubMed id: 21600989

Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily.

S.J.Lee, D.J.Kim, H.S.Kim, B.I.Lee, H.J.Yoon, J.Y.Yoon, K.H.Kim, J.Y.Jang, H.N.Im, D.R.An, J.S.Song, H.J.Kim, S.W.Suh.

ABSTRACT

No abstract given.