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PDBsum entry 3nh2

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protein dna_rna Protein-protein interface(s) links
Hydrolase/DNA PDB id
3nh2

 

 

 

 

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Contents
Protein chains
202 a.a. *
DNA/RNA
Waters ×345
* Residue conservation analysis
PDB id:
3nh2
Name: Hydrolase/DNA
Title: Crystal structure of rnase t in complex with a stem DNA with a 3' overhang
Structure: Ribonuclease t. Chain: a, b, e, f. Synonym: rnase t, exoribonuclease t. Engineered: yes. 5'-d(p Tp Tp Ap Cp Ap Ap C)-3'. Chain: c, d, g, h. Engineered: yes. Other_details: ssdna
Source: Escherichia coli. Organism_taxid: 562. Strain: jm109 / atcc 53323. Gene: rnt. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630
Resolution:
2.30Å     R-factor:   0.190     R-free:   0.241
Authors: Y.-Y.Hsiao,H.S.Yuan
Key ref: Y.Y.Hsiao et al. (2011). Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation. Nat Chem Biol, 7, 236-243. PubMed id: 21317904
Date:
14-Jun-10     Release date:   16-Feb-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P30014  (RNT_ECOLI) -  Ribonuclease T from Escherichia coli (strain K12)
Seq:
Struc:
215 a.a.
202 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

DNA/RNA chains
  T-T-A-C-A-A-C 7 bases
  T-A-C-A-A-C 6 bases
  T-A-C-A-A-C 6 bases
  T-T-A-C-A-A-C 7 bases

 Enzyme reactions 
   Enzyme class: E.C.3.1.13.-  - ?????
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Nat Chem Biol 7:236-243 (2011)
PubMed id: 21317904  
 
 
Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation.
Y.Y.Hsiao, C.C.Yang, C.L.Lin, J.L.Lin, Y.Duh, H.S.Yuan.
 
  ABSTRACT  
 
No abstract given.

 

 

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