PDBsum entry 3n0h

Oxidoreductase

PDB id: 3n0h

Contents

Protein chain

186 a.a.

Ligands

TOP ×2

SO4 ×3

Waters ×91

PDB id:

3n0h

Name:

Oxidoreductase

Title:

Hdhfr double mutant q35s/n64f trimethoprim binary complex

Structure:

Dihydrofolate reductase. Chain: a. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: dhfr, dhfrp1, pds5. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.92Å R-factor: 0.200 R-free: 0.251

Authors:

V.Cody

Key ref:

V.Cody et al. (2011). Crystallographic analysis reveals a novel second binding site for trimethoprim in active site double mutants of human dihydrofolate reductase. J Struct Biol, 176, 52-59. PubMed id: 21684339

Date:

14-May-10 Release date: 28-Sep-11

Protein chain

P00374  (DYR_HUMAN) -  Dihydrofolate reductase from Homo sapiens

Seq: 187 a.a.

Struc: 186 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Struct Biol 176:52-59 (2011)

PubMed id: 21684339

Crystallographic analysis reveals a novel second binding site for trimethoprim in active site double mutants of human dihydrofolate reductase.

V.Cody, J.Pace, J.Piraino, S.F.Queener.

ABSTRACT

No abstract given.