PDBsum entry 3n0c

Transferase

PDB id: 3n0c

Contents

Protein chains

216 a.a.

Ligands

UMP ×4

FAD ×4

Waters ×46

PDB id:

3n0c

Name:

Transferase

Title:

Tm0449 mutant crystal grown by hanging drop method

Structure:

Thymidylate synthase thyx. Chain: a, b, c, d. Synonym: ts, tsase. Engineered: yes. Mutation: yes

Source:

Thermotoga maritima. Organism_taxid: 2336. Gene: thy1, thyx, tm0449, tm_0449. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.30Å R-factor: 0.196 R-free: 0.248

Authors:

I.I.Mathews

Key ref:

M.A.Berger et al. (2010). Diffraction study of protein crystals grown in cryoloops and micromounts. J Appl Crystallogr, 43, 1513-1518. PubMed id: 22477781

Date:

13-May-10 Release date: 25-May-11

Protein chains

Q9WYT0  (THYX_THEMA) -  Flavin-dependent thymidylate synthase from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

Seq: 220 a.a.

Struc: 216 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.1.1.148 - thymidylate synthase (FAD).
• Reaction: dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H⁺ = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP⁺

dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (Bound ligand (Het Group name = UMP) corresponds exactly) + NADPH + H(+) (Bound ligand (Het Group name = FAD) matches with 71.19% similarity) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Appl Crystallogr 43:1513-1518 (2010)

PubMed id: 22477781

Diffraction study of protein crystals grown in cryoloops and micromounts.

M.A.Berger, J.H.Decker, I.I.Mathews.

ABSTRACT

No abstract given.