 |
PDBsum entry 3mzf
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Hydrolase/antibiotic
|
PDB id
|
|
|
|
3mzf
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase/antibiotic
|
|
|
Title:
|
|
Structure of penicillin-binding protein 5 from e. Coli: imipenem acyl- enzyme complex
|
|
Structure:
|
|
D-alanyl-d-alanine carboxypeptidase daca. Chain: a. Fragment: soluble construct. Synonym: dd-carboxypeptidase, dd-peptidase, beta-lactamase, penicillin-binding protein 5, pbp-5. Engineered: yes
|
|
Source:
|
|
Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b0632, daca, jw0627, pfv. Expressed in: escherichia coli. Expression_system_taxid: 562.
|
|
Resolution:
|
|
|
1.50Å
|
R-factor:
|
0.189
|
R-free:
|
0.212
|
|
|
Authors:
|
|
G.Nicola,J.Tomberg,R.F.Pratt,R.A.Nicholas,C.Davies
|
|
Key ref:
|
|
G.Nicola
et al.
(2010).
Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity.
Biochemistry,
49,
8094-8104.
PubMed id:
|
|
|
Date:
|
|
|
12-May-10
|
Release date:
|
16-Mar-11
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P0AEB2
(DACA_ECOLI) -
D-alanyl-D-alanine carboxypeptidase DacA from Escherichia coli (strain K12)
|
|
|
|
Seq:
Struc:
|
|
|
|
403 a.a.
355 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class 1:
|
|
E.C.3.4.16.4
- serine-type D-Ala-D-Ala carboxypeptidase.
|
|
|
|
|
|
|
Reaction:
|
|
D-alanyl-D-alanine + H2O = 2 D-alanine
|
|
|
|
|
|
|
+
|
|
=
|
2
×
|
|
|
|
|
|
|
|
|
|
Enzyme class 2:
|
|
E.C.3.5.2.6
- beta-lactamase.
|
|
|
|
|
|
|
Pathway:
|
|
|
|
|
|
|
|
Reaction:
|
|
a beta-lactam + H2O = a substituted beta-amino acid
|
|
|
|
|
|
Cofactor:
|
|
Zn(2+)
|
|
|
|
|
|
|
|
|
Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
|
|
|
|
Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
| |
|
|
Biochemistry
49:8094-8104
(2010)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity.
|
|
G.Nicola,
J.Tomberg,
R.F.Pratt,
R.A.Nicholas,
C.Davies.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
