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PDBsum entry 3mzd
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Hydrolase/antibiotic
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PDB id
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3mzd
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PDB id:
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| Name: |
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Hydrolase/antibiotic
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Title:
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Structure of penicillin-binding protein 5 from e. Coli: cloxacillin acyl-enzyme complex
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Structure:
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D-alanyl-d-alanine carboxypeptidase daca. Chain: a. Fragment: soluble construct. Synonym: dd-carboxypeptidase, dd-peptidase, beta-lactamase, penicillin-binding protein 5, pbp-5. Engineered: yes
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Source:
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Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b0632, daca, jw0627, pfv. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.90Å
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R-factor:
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0.216
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R-free:
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0.257
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Authors:
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G.Nicola,J.Tomberg,R.F.Pratt,R.A.Nicholas,C.Davies
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Key ref:
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G.Nicola
et al.
(2010).
Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity.
Biochemistry,
49,
8094-8104.
PubMed id:
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Date:
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12-May-10
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Release date:
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16-Mar-11
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PROCHECK
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Headers
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References
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P0AEB2
(DACA_ECOLI) -
D-alanyl-D-alanine carboxypeptidase DacA from Escherichia coli (strain K12)
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Seq:
Struc:
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403 a.a.
354 a.a.
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Key: |
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Secondary structure |
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CATH domain |
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Enzyme class 1:
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E.C.3.4.16.4
- serine-type D-Ala-D-Ala carboxypeptidase.
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Reaction:
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D-alanyl-D-alanine + H2O = 2 D-alanine
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+
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=
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2
×
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Enzyme class 2:
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E.C.3.5.2.6
- beta-lactamase.
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Pathway:
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Reaction:
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a beta-lactam + H2O = a substituted beta-amino acid
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Cofactor:
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Zn(2+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Biochemistry
49:8094-8104
(2010)
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PubMed id:
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Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity.
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G.Nicola,
J.Tomberg,
R.F.Pratt,
R.A.Nicholas,
C.Davies.
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ABSTRACT
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