PDBsum entry 3mz0

Oxidoreductase

PDB id: 3mz0

Contents

Protein chain

337 a.a. *

Ligands

PGE ×2

GOL

Metals

_MG

_CL ×4

Waters ×375

* Residue conservation analysis

PDB id:

3mz0

Name:

Oxidoreductase

Title:

Crystal structure of apo myo-inositol dehydrogenase from bacillus subtilis

Structure:

Inositol 2-dehydrogenase/d-chiro-inositol 3-dehydrogenase. Chain: a. Synonym: myo-inositol 2-dehydrogenase/d-chiro-inositol 3- dehydrogenase, mi 2-dehydrogenase/dci 3-dehydrogenase. Engineered: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Gene: bsu39700, e83g, idh, iolg, np_391849.2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.54Å R-factor: 0.176 R-free: 0.194

Authors:

K.E.Van Straaten,D.R.J.Palmer,D.A.R.Sanders

Key ref:

K.E.van Straaten et al. (2010). Structural investigation of myo-inositol dehydrogenase from Bacillus subtilis: implications for catalytic mechanism and inositol dehydrogenase subfamily classification. Biochem J, 432, 237-247. PubMed id: 20809899

Date:

11-May-10 Release date: 29-Sep-10

Protein chain

P26935  (IOLG_BACSU) -  Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase from Bacillus subtilis (strain 168)

Seq: 344 a.a.

Struc: 337 a.a.

Enzyme reactions

• Enzyme class 1: E.C.1.1.1.18 - inositol 2-dehydrogenase.
• Reaction: myo-inositol + NAD⁺ = scyllo-inosose + NADH + H⁺

myo-inositol (Bound ligand (Het Group name = GOL) matches with 50.00% similarity) + NAD(+) = scyllo-inosose + NADH + H(+)

• Enzyme class 2: E.C.1.1.1.369 - D-chiro-inositol 1-dehydrogenase.
• Reaction: 1D-chiro-inositol + NAD⁺ = scyllo-inosine + NADH + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochem J 432:237-247 (2010)

PubMed id: 20809899

Structural investigation of myo-inositol dehydrogenase from Bacillus subtilis: implications for catalytic mechanism and inositol dehydrogenase subfamily classification.

K.E.van Straaten, H.Zheng, D.R.Palmer, D.A.Sanders.

ABSTRACT

No abstract given.