PDBsum entry 3mwc

Ligase

PDB id: 3mwc

Contents

Protein chain

388 a.a. *

Ligands

PO4

Metals

_MG

Waters ×170

* Residue conservation analysis

PDB id:

3mwc

Name:

Ligase

Title:

Crystal structure of probable o-succinylbenzoic acid synthetase from kosmotoga olearia

Structure:

Mandelate racemase/muconate lactonizing protein. Chain: a. Synonym: probable o-succinylbenzoic acid synthetase. Engineered: yes

Source:

Kosmotoga olearia. Organism_taxid: 521045. Strain: tbf 19.5.1. Gene: kole_0682. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.80Å R-factor: 0.210 R-free: 0.262

Authors:

Y.Patskovsky,R.Toro,M.Dickey,J.M.Sauder,J.Gerlt,S.C.Almo,S.K.Burley, New York Sgx Research Center For Structural Genomics (Nysgxrc)

Key ref:

Y.Patskovsky et al. Crystal structure of o-Succinylbenzoic acid synthetas kosmotoga olearia. To be published, .

Date:

05-May-10 Release date: 19-May-10

Protein chain

C5CFI0  (C5CFI0_KOSOT) -  o-succinylbenzoate synthase from Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1)

Seq: 391 a.a.

Struc: 388 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.1.113 - o-succinylbenzoate synthase.
• Reaction: (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O
• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site