PDBsum entry 3mpp

Toxin

PDB id

3mpp

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Contents

			Protein chain

					403 a.a. *

			Waters ×332

* Residue conservation analysis

PDB id:

3mpp

Links

Name:

Toxin

Title:

Botulinum neurotoxin type g receptor binding domain

Structure:

Botulinum neurotoxin type g. Chain: g. Fragment: receptor binding domain (unp residues 867:1297). Synonym: bont/g, bontoxilysin-g, botulinum neurotoxin g light chain, botulinum neurotoxin g heavy chain. Engineered: yes

Source:

Clostridium botulinum. Organism_taxid: 1491. Gene: botg. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.98Å

R-factor:

0.177

R-free:

0.221

Authors:

J.M.Schmitt,D.B.Lacy

Key ref:

J.Schmitt et al. (2010). Structural analysis of botulinum neurotoxin type G receptor binding . Biochemistry, 49, 5200-5205. PubMed id: 20507178

Date:

27-Apr-10

Release date:

16-Jun-10

PROCHECK

	Headers

	References

Protein chain

Q60393 (BXG_CLOBO) - Botulinum neurotoxin type G from Clostridium botulinum

Seq: Struc:

Seq: Struc:

Seq: Struc:					1297 a.a. 403 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 4 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.3.4.24.69 - bontoxilysin.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.

Cofactor:

Zn(2+)

	Biochemistry 49:5200-5205 (2010)
PubMed id: 20507178

Structural analysis of botulinum neurotoxin type G receptor binding .
J.Schmitt, A.Karalewitz, D.A.Benefield, D.J.Mushrush, R.N.Pruitt, B.W.Spiller, J.T.Barbieri, D.B.Lacy.

ABSTRACT

Botulinum neurotoxin (BoNT) binds peripheral neurons at the neuromuscular junction through a dual-receptor mechanism that includes interactions with ganglioside and protein receptors. The receptor identities vary depending on BoNT serotype (A-G). BoNT/B and BoNT/G bind the luminal domains of synaptotagmin I and II, homologous synaptic vesicle proteins. We observe conditions under which BoNT/B binds both Syt isoforms, but BoNT/G binds only SytI. Both serotypes bind ganglioside G(T1b). The BoNT/G receptor-binding domain crystal structure provides a context for examining these binding interactions and a platform for understanding the physiological relevance of different Syt receptor isoforms in vivo.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20704566

J.Strotmeier, K.Lee, A.K.Völker, S.Mahrhold, Y.Zong, J.Zeiser, J.Zhou, A.Pich, H.Bigalke, T.Binz, A.Rummel, and R.Jin (2010).
Botulinum neurotoxin serotype D attacks neurons via two carbohydrate-binding sites in a ganglioside-dependent manner.

Biochem J, 431, 207-216.

PDB codes:

3obr 3obt

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.