PDBsum entry 3moj

RNA binding protein/RNA

PDB id

3moj

Loading ...

Contents

			Protein chain

					75 a.a. *

			DNA/RNA

* Residue conservation analysis

PDB id:

3moj

Links

Name:

RNA binding protein/RNA

Title:

Structure of the RNA binding domain of the bacillus subtilis yxin protein complexed with a fragment of 23s ribosomal RNA

Structure:

RNA (69-mer). Chain: a. Fragment: e. Coli 23 s ribosomal RNA (nucleotides 2508-2580 with gaaa tetraloop in nucleotides 2530-2533 and a2581 at 3' end). Engineered: yes. Atp-dependent RNA helicase dbpa. Chain: b. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562. Bacillus subtilis. Organism_taxid: 1423. Gene: dbpa, dead, yxin, bsu39110, ss8e.

Resolution:

2.90Å

R-factor:

0.216

R-free:

0.275

Authors:

J.W.Hardin,Y.Hu,D.B.Mckay

Key ref:

J.W.Hardin et al. (2010). Structure of the RNA binding domain of a DEAD-box helicase bound to its ribosomal RNA target reveals a novel mode of recognition by an RNA recognition motif. J Mol Biol, 402, 412-427. PubMed id: 20673833

Date:

22-Apr-10

Release date:

04-Aug-10

PROCHECK

	Headers

	References

Protein chain

P42305 (DBPA_BACSU) - ATP-dependent RNA helicase DbpA from Bacillus subtilis (strain 168)

Seq: Struc:					479 a.a. 75 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

DNA/RNA chain

G-G-C-U-C-A-U-C-A-C-A-U-C-C-U-G-G-G-G-C-U-G-G-A-A-A-C-G-G-U-C-C-C-A-A-G-G-G-U- 69 bases



		Enzyme reactions

Enzyme class:

E.C.3.6.4.13 - Rna helicase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

ATP + H2O = ADP + phosphate + H⁺

ATP	+	H2O	=	ADP	+	phosphate	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	J Mol Biol 402:412-427 (2010)
PubMed id: 20673833

Structure of the RNA binding domain of a DEAD-box helicase bound to its ribosomal RNA target reveals a novel mode of recognition by an RNA recognition motif.
J.W.Hardin, Y.X.Hu, D.B.McKay.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21428949

D.Klostermeier (2011).
Single-molecule FRET reveals nucleotide-driven conformational changes in molecular machines and their link to RNA unwinding and DNA supercoiling.

Biochem Soc Trans, 39, 611-616.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.