PDBsum entry 3mjs

Oxidoreductase

PDB id

3mjs

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Contents

			Protein chains

					476 a.a. *

			Ligands

					NDP ×2


					LMR


					MLT ×3


					GOL ×2

			Waters ×504

* Residue conservation analysis

PDB id:

3mjs

Links

Name:

Oxidoreductase

Title:

Structure of a-type ketoreductases from modular polyketide synthase

Structure:

Amphb. Chain: a, b. Fragment: ketoreductase domain. Engineered: yes

Source:

Streptomyces nodosus. Organism_taxid: 40318. Gene: amphb. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.40Å

R-factor:

0.200

R-free:

0.226

Authors:

J.Zheng,C.A.Taylor,S.K.Piasecki,A.T.Keatinge-Clay

Key ref:

J.Zheng et al. (2010). Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase. Structure, 18, 913-922. PubMed id: 20696392

Date:

13-Apr-10

Release date:

18-Aug-10

PROCHECK

	Headers

	References

Protein chains

Q93NW7 (Q93NW7_9ACTN) - AmphB from Streptomyces nodosus

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					3190 a.a. 476 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a (3R)-hydroxyacyl-[ACP] + NADP⁺ = a 3-oxoacyl-[ACP] + NADPH + H⁺

(3R)-hydroxyacyl-[ACP]

NADP(+)
Bound ligand (Het Group name = NDP)
corresponds exactly

3-oxoacyl-[ACP]

NADPH

H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Structure 18:913-922 (2010)
PubMed id: 20696392

Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase.
J.Zheng, C.A.Taylor, S.K.Piasecki, A.T.Keatinge-Clay.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21340070

D.H.Kwan, M.Tosin, N.Schläger, F.Schulz, and P.F.Leadlay (2011).
Insights into the stereospecificity of ketoreduction in a modular polyketide synthase.

Org Biomol Chem, 9, 2053-2056.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.