PDBsum entry 3lx0

Hydrolase

PDB id: 3lx0

Contents

Protein chain

136 a.a. *

Ligands

THP

PO4

Waters ×142

* Residue conservation analysis

PDB id:

3lx0

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs d21n at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: residues 83-231, deletion 126-131. Synonym: tnase, micrococcal nuclease, staphylococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: sfag_02204. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.50Å R-factor: 0.183 R-free: 0.222

Authors:

B.M.Doctrow,J.L.Schlessman,E.B.Garcia-Moreno,A.Heroux

Key ref:

B.M.Doctrow et al. Cooperative proton binding in a cluster of carboxylic residues. To be published, .

Date:

24-Feb-10 Release date: 16-Feb-11

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 136 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.