PDBsum entry 3l2c

Transcription/DNA

PDB id: 3l2c

Contents

Protein chain

85 a.a. *

DNA/RNA

Metals

_MG ×2

Waters ×163

* Residue conservation analysis

PDB id:

3l2c

Name:

Transcription/DNA

Title:

Crystal structure of the DNA binding domain of foxo4 bound to DNA

Structure:

Foxo consensus binding sequence, plus strand. Chain: b. Engineered: yes. Foxo consensus binding sequence, minus strand. Chain: c. Engineered: yes. Forkhead box protein o4. Chain: a. Fragment: DNA binding domain, unp residues 86-187.

Source:

Synthetic: yes. Other_details: synthetic DNA. Homo sapiens. Human. Organism_taxid: 9606. Gene: afx, afx1, foxo4, mllt7. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.87Å R-factor: 0.195 R-free: 0.228

Authors:

E.Boura,J.Silhan,M.Sulc,J.Brynda,V.Obsilova,T.Obsil

Key ref:

E.Boura et al. (2010). Structure of the human FOXO4-DBD-DNA complex at 1.9 Å resolution reveals new details of FOXO binding to the DNA. Acta Crystallogr D Biol Crystallogr, 66, 1351-1357. PubMed id: 21123876

Date:

15-Dec-09 Release date: 29-Dec-09

Supersedes:

3bpy

Protein chain

P98177  (FOXO4_HUMAN) -  Forkhead box protein O4 from Homo sapiens

Seq: 505 a.a.

Struc: 85 a.a.

DNA/RNA chains

C-T-A-T-G-T-A-A-A-C-A-A-C 13 bases

G-T-T-G-T-T-T-A-C-A-T-A-G 13 bases

Enzyme reactions

• Enzyme class: E.C.?

Acta Crystallogr D Biol Crystallogr 66:1351-1357 (2010)

PubMed id: 21123876

Structure of the human FOXO4-DBD-DNA complex at 1.9 Å resolution reveals new details of FOXO binding to the DNA.

E.Boura, L.Rezabkova, J.Brynda, V.Obsilova, T.Obsil.

ABSTRACT

No abstract given.