PDBsum entry 3l1e

Chaperone

PDB id

3l1e

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Contents

			Protein chain

					105 a.a. *

			Ligands

					GOL

			Metals

					_ZN

			Waters ×95

* Residue conservation analysis

PDB id:

3l1e

Links

Name:

Chaperone

Title:

Bovine alphaa crystallin zinc bound

Structure:

Alpha-crystallin a chain. Chain: a. Fragment: residues 59-163. Synonym: alpha-crystallin a chain, short form. Engineered: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Gene: crya1, cryaa. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.15Å

R-factor:

0.166

R-free:

0.193

Authors:

A.Laganowsky,M.R.Sawaya,D.Cascio,D.Eisenberg

Key ref:

A.Laganowsky et al. (2010). Crystal structures of truncated alphaA and alphaB crystallins reveal structural mechanisms of polydispersity important for eye lens function. Protein Sci, 19, 1031-1043. PubMed id: 20440841

Date:

11-Dec-09

Release date:

12-May-10

PROCHECK

	Headers

	References

Protein chain

P02470 (CRYAA_BOVIN) - Alpha-crystallin A chain from Bos taurus

Seq: Struc:					173 a.a. 105 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Protein Sci 19:1031-1043 (2010)
PubMed id: 20440841

Crystal structures of truncated alphaA and alphaB crystallins reveal structural mechanisms of polydispersity important for eye lens function.
A.Laganowsky, J.L.Benesch, M.Landau, L.Ding, M.R.Sawaya, D.Cascio, Q.Huang, C.V.Robinson, J.Horwitz, D.Eisenberg.

ABSTRACT

Small heat shock proteins alphaA and alphaB crystallin form highly polydisperse oligomers that frustrate protein aggregation, crystallization, and amyloid formation. Here, we present the crystal structures of truncated forms of bovine alphaA crystallin (AAC(59-163)) and human alphaB crystallin (ABC(68-162)), both containing the C-terminal extension that functions in chaperone action and oligomeric assembly. In both structures, the C-terminal extensions swap into neighboring molecules, creating runaway domain swaps. This interface, termed DS, enables crystallin polydispersity because the C-terminal extension is palindromic and thereby allows the formation of equivalent residue interactions in both directions. That is, we observe that the extension binds in opposite directions at the DS interfaces of AAC(59-163) and ABC(68-162). A second dimeric interface, termed AP, also enables polydispersity by forming an antiparallel beta sheet with three distinct registration shifts. These two polymorphic interfaces enforce polydispersity of alpha crystallin. This evolved polydispersity suggests molecular mechanisms for chaperone action and for prevention of crystallization, both necessary for transparency of eye lenses.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21329698

A.R.Clark, C.E.Naylor, C.Bagnéris, N.H.Keep, and C.Slingsby (2011).
Crystal structure of R120G disease mutant of human αB-crystallin domain dimer shows closure of a groove.

J Mol Biol, 408, 118-134.

PDB codes:

2y1y 2y1z 2y22

21315085

E.Hilario, F.J.Martin, M.C.Bertolini, and L.Fan (2011).
Crystal structures of Xanthomonas small heat shock protein provide a structural basis for an active molecular chaperone oligomer.

J Mol Biol, 408, 74-86.

PDB codes:

3gt6 3guf

21195185

K.Takeda, T.Hayashi, T.Abe, Y.Hirano, Y.Hanazono, M.Yohda, and K.Miki (2011).
Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein, StHsp14.0.

J Struct Biol, 174, 92-99.

PDB codes:

3aab 3aac

21300147

M.Raju, P.Santhoshkumar, T.M.Henzl, and K.K.Sharma (2011).
Identification and characterization of a copper-binding site in αA-crystallin.

Free Radic Biol Med, 50, 1429-1436.

21173272

P.R.Banerjee, A.Pande, J.Patrosz, G.M.Thurston, and J.Pande (2011).
Cataract-associated mutant E107A of human gammaD-crystallin shows increased attraction to alpha-crystallin and enhanced light scattering.

Proc Natl Acad Sci U S A, 108, 574-579.

21464278

S.Jehle, B.S.Vollmar, B.Bardiaux, K.K.Dove, P.Rajagopal, T.Gonen, H.Oschkinat, and R.E.Klevit (2011).
N-terminal domain of alphaB-crystallin provides a conformational switch for multimerization and structural heterogeneity.

Proc Natl Acad Sci U S A, 108, 6409-6414.

20857505

S.Karmakar, and K.P.Das (2011).
Stabilization of oligomeric structure of α-crystallin by Zn(+2) through intersubunit bridging.

Biopolymers, 95, 105-116.

20669149

A.Laganowsky, and D.Eisenberg (2010).
Non-3D domain swapped crystal structure of truncated zebrafish alphaA crystallin.

Protein Sci, 19, 1978-1984.

PDB code:

3n3e

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.