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PDBsum entry 3keh
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Contents |
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* Residue conservation analysis
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PDB id:
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Hydrolase
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Title:
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Crystal structure of n370s glucocerebrosidase mutant at ph 7.4
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Structure:
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Glucocerebrosidase. Chain: a, b. Synonym: beta-glucocerebrosidase, acid beta-glucosidase, d-glucosyl- n-acylsphingosine glucohydrolase, alglucerase, imiglucerase. Engineered: yes. Mutation: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108
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Resolution:
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2.80Å
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R-factor:
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0.215
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R-free:
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0.273
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Authors:
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R.R.Wei,S.Boucher,C.Q.Pan,T.Edmunds
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Key ref:
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R.R.Wei
et al.
Crystal structure of glucocerebrosidase containing th mutation: implication on chaperon therapy.
To be published,
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Date:
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26-Oct-09
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Release date:
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27-Oct-10
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PROCHECK
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Headers
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References
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P04062
(GLCM_HUMAN) -
Lysosomal acid glucosylceramidase from Homo sapiens
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Seq:
Struc:
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536 a.a.
497 a.a.*
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Key: |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 2:
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E.C.2.4.1.-
- ?????
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Enzyme class 3:
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E.C.3.2.1.-
- ?????
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Enzyme class 4:
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E.C.3.2.1.45
- glucosylceramidase.
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Reaction:
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a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing- 4-enine + D-glucose
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beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine
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+
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H2O
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=
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N-acylsphing- 4-enine
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+
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D-glucose
Bound ligand (Het Group name = )
matches with 62.50% similarity
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Enzyme class 5:
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E.C.3.2.1.46
- galactosylceramidase.
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Reaction:
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a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-galactose
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beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine
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+
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H2O
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=
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N-acylsphing-4-enine
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+
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D-galactose
Bound ligand (Het Group name = )
matches with 62.50% similarity
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
