PDBsum entry 3js3

Lyase

PDB id: 3js3

Contents

Protein chains

253 a.a. *

Ligands

DHS ×4

Waters ×322

* Residue conservation analysis

PDB id:

3js3

Name:

Lyase

Title:

Crystal structure of type i 3-dehydroquinate dehydratase (arod) from clostridium difficile with covalent reaction intermediate

Structure:

3-dehydroquinate dehydratase. Chain: a, b, c, d. Synonym: 3-dehydroquinase, type i dhqase. Engineered: yes

Source:

Clostridium difficile. Organism_taxid: 272563. Strain: 630. Gene: arod, cd2217. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.192 R-free: 0.241

Authors:

G.Minasov,S.H.Light,L.Shuvalova,I.Dubrovska,J.Winsor,S.N.Peterson, W.F.Anderson,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

S.H.Light et al. (2011). Insights into the mechanism of type I dehydroquinate dehydratases from structures of reaction intermediates. J Biol Chem, 286, 3531-3539. PubMed id: 21087925

Date:

09-Sep-09 Release date: 22-Sep-09

Protein chains

Q186A6  (AROD_PEPD6) -  3-dehydroquinate dehydratase from Clostridioides difficile (strain 630)

Seq: 255 a.a.

Struc: 253 a.a.

Enzyme reactions

• Enzyme class: E.C.4.2.1.10 - 3-dehydroquinate dehydratase.
• Pathway: Shikimate and Chorismate Biosynthesis
• Reaction: 3-dehydroquinate = 3-dehydroshikimate + H2O

3-dehydroquinate = 3-dehydroshikimate (Bound ligand (Het Group name = DHS) matches with 91.67% similarity) + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 286:3531-3539 (2011)

PubMed id: 21087925

Insights into the mechanism of type I dehydroquinate dehydratases from structures of reaction intermediates.

S.H.Light, G.Minasov, L.Shuvalova, M.E.Duban, M.Caffrey, W.F.Anderson, A.Lavie.

ABSTRACT

No abstract given.