 |
PDBsum entry 3iv2
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase
|
|
|
Title:
|
|
Crystal structure of mature apo-cathepsin l c25a mutant
|
|
Structure:
|
|
Cathepsin l1. Chain: a, b. Fragment: mature protein: unp residues 114-333. Synonym: major excreted protein, mep, cathepsin l1 heavy chain and cathepsin l1 light chain. Engineered: yes. Mutation: yes
|
|
Source:
|
|
Homo sapiens. Human. Organism_taxid: 9606. Gene: ctsl1, ctsl. Expressed in: pichia pastoris. Expression_system_taxid: 4922
|
|
Resolution:
|
|
|
2.20Å
|
R-factor:
|
0.204
|
R-free:
|
0.244
|
|
|
Authors:
|
|
M.A.Adams-Cioaba,J.C.Krupa,J.S.Mort,C.Bountra,J.Weigelt, C.H.Arrowsmith,A.M.Edwards,A.Bochkarev,J.Min
|
|
Key ref:
|
|
M.A.Adams-Cioaba
et al.
(2011).
Structural basis for the recognition and cleavage of histone H3 by cathepsin L.
Nat Commun,
2,
197-197.
PubMed id:
|
|
|
Date:
|
|
|
31-Aug-09
|
Release date:
|
23-Mar-10
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P07711
(CATL1_HUMAN) -
Procathepsin L from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
333 a.a.
219 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
E.C.3.4.22.15
- cathepsin L.
|
|
|
|
|
|
|
Reaction:
|
|
Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Nat Commun
2:197-197
(2011)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Structural basis for the recognition and cleavage of histone H3 by cathepsin L.
|
|
M.A.Adams-Cioaba,
J.C.Krupa,
C.Xu,
J.S.Mort,
J.Min.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
