PDBsum entry 3itp

Hydrolase

PDB id: 3itp

Contents

Protein chain

129 a.a. *

Ligands

THP

Metals

_CA

Waters ×72

* Residue conservation analysis

PDB id:

3itp

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs f34k at cryogenic temperature

Structure:

Nuclease a. Chain: a. Fragment: unp residues 83-231. Synonym: tnase, micrococcal nuclease, staphylococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: nuc. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.75Å R-factor: 0.204 R-free: 0.235

Authors:

V.S.Khangulov,J.L.Schlessman,A.Heroux,E.B.Garcia-Moreno

Key ref:

V.S.Khangulov et al. Crystal structure of staphylococcal nuclease variant delta+phs f34k at cryogenic temperature. To be published, .

Date:

28-Aug-09 Release date: 21-Jul-10

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.