PDBsum entry 3gci

Hydrolase

PDB id: 3gci

Contents

Protein chain

119 a.a. *

Ligands

VAL-GLY-GLY-VAL-VAL-ILE-ALA

Metals

_CA

Waters ×93

* Residue conservation analysis

PDB id:

3gci

Name:

Hydrolase

Title:

Crystal structure of the complex formed between a new isoform of phospholipase a2 with c-terminal amyloid beta heptapeptide at 2 a resolution

Structure:

Phospholipase a2 isoform 3. Chain: a. Synonym: phosphatidylcholine 2-acylhydrolase. Heptapeptide from amyloid beta a4 protein. Chain: p. Fragment: unp residues 707-713. Engineered: yes

Source:

Naja sagittifera. Andaman cobra. Organism_taxid: 195058. Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Other_details: this peptide was chemically synthesized. This sequence occurs naturally in humans.

Resolution:

2.04Å R-factor: 0.186 R-free: 0.221

Authors:

Z.Mirza,G.Vikram,N.Singh,M.Sinha,A.Bhushan,S.Sharma,A.Srinivasan, P.Kaur,T.P.Singh

Key ref:

Z.Mirza et al. Crystal structure of the complex formed between a new isoform of phospholipase a2 with c-Terminal amyloid beta heptapeptide at 2 a resolution. To be published, .

Date:

22-Feb-09 Release date: 10-Mar-09

Protein chain

P60045  (PA2A3_NAJSG) -  Acidic phospholipase A2 3 (Fragment) from Naja sagittifera

Seq: 126 a.a.

Struc: 119 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.1.4 - phospholipase A2.
• Reaction: a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3- phosphocholine + a fatty acid + H⁺

1,2-diacyl-sn-glycero-3-phosphocholine + H2O = 1-acyl-sn-glycero-3- phosphocholine + fatty acid (Bound ligand (Het Group name = ALA) matches with 42.86% similarity) + H(+)

• Cofactor: Ca(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site