PDBsum entry 3fpy

Electron transport

PDB id: 3fpy

Contents

Protein chain

128 a.a. *

Ligands

TRS

Metals

_CU ×2

Waters ×175

* Residue conservation analysis

PDB id:

3fpy

Name:

Electron transport

Title:

Azurin c112d/m121l

Structure:

Azurin. Chain: a. Engineered: yes. Mutation: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Gene: azu, pa4922. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.178 R-free: 0.236

Authors:

K.M.Lancaster,H.B.Gray

Key ref:

K.M.Lancaster et al. (2009). Type-zero copper proteins. Nat Chem, 1, 711-715. PubMed id: 20305734

Date:

06-Jan-09 Release date: 10-Nov-09

Protein chain

P00282  (AZUR_PSEAE) -  Azurin from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 148 a.a.

Struc: 128 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Nat Chem 1:711-715 (2009)

PubMed id: 20305734

Type-zero copper proteins.

K.M.Lancaster, S.DeBeer George, K.Yokoyama, J.H.Richards, H.B.Gray.

ABSTRACT

Copper proteins play key roles in biological processes such as electron transfer and dioxygen activation; the active site of each of these proteins is classified as either type 1, 2, or 3, depending on its optical and electron paramagnetic resonance properties. We have built a new type of site that we call "type zero copper" by incorporating leucine, isoleucine, or phenylalanine in place of methionine at position 121 in C112D Pseudomonas aeruginosa azurin. X-ray crystallographic analysis shows that these sites adopt distorted tetrahedral geometries, with an unusually short Cu-O(G45 carbonyl) bond (2.35-2.55 A). Relatively weak absorption near 800 nm and narrow parallel hyperfine splittings in EPR spectra are the spectroscopic signatures of type zero copper. Copper K-edge x-ray absorption spectra suggest elevated Cu(II) 4p character in the d-electron ground state. Cyclic voltammetric experiments demonstrate that the electron transfer reactivities of type zero azurins are enhanced relative to that of the corresponding type 2 (C112D) protein.