PDBsum entry 3f2e

Viral protein

PDB id: 3f2e

Contents

Protein chain

83 a.a.

Ligands

CIT

Waters ×80

PDB id:

3f2e

Name:

Viral protein

Title:

Crystal structure of yellowstone sirv coat protein c-terminus

Structure:

Sirv coat protein. Chain: a. Fragment: c-terminal domain. Engineered: yes

Source:

Sulfolobus islandicus rudivirus 1 variant ynp. Organism_taxid: 187213. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.67Å R-factor: 0.185 R-free: 0.202

Authors:

R.E.Taurog,B.R.Szymczyna,J.R.Williamson,J.E.Johnson

Key ref:

B.R.Szymczyna et al. (2009). Synergy of NMR, computation, and X-ray crystallography for structural biology. Structure, 17, 499-507. PubMed id: 19368883

Date:

29-Oct-08 Release date: 21-Apr-09

Protein chain

D0VX05  (D0VX05_9VIRU) -  SIRV coat protein from Rudivirus

Seq: 100 a.a.

Struc: 83 a.a.

Enzyme reactions

• Enzyme class: E.C.?

Structure 17:499-507 (2009)

PubMed id: 19368883

Synergy of NMR, computation, and X-ray crystallography for structural biology.

B.R.Szymczyna, R.E.Taurog, M.J.Young, J.C.Snyder, J.E.Johnson, J.R.Williamson.

ABSTRACT

NMR spectroscopy and X-ray crystallography are currently the two most widely applied methods for the determination of macromolecular structures at high resolution. More recently, significant advances have been made in algorithms for the de novo prediction of protein structure, and, in favorable cases, the predicted models agree extremely well with experimentally determined structures. Here, we demonstrate a synergistic combination of NMR spectroscopy, de novo structure prediction, and X-ray crystallography in an effective overall strategy for rapidly determining the structure of the coat protein C-terminal domain from the Sulfolobus islandicus rod-shaped virus (SIRV). This approach takes advantage of the most accessible aspects of each structural technique and may be widely applicable for structure determination.