PDBsum entry 3f0e

Lyase

PDB id

3f0e

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Contents

			Protein chains

					158 a.a. *


					146 a.a. *

			Metals

					_ZN ×3


					_MG

			Waters ×249

* Residue conservation analysis

PDB id:

3f0e

Links

Name:

Lyase

Title:

Crystal structure of 2c-methyl-d-erythritol 2,4-cyclodiphosphate synthase from burkholderia pseudomallei

Structure:

2-c-methyl-d-erythritol 2,4-cyclodiphosphate synthase. Chain: a, b, c. Synonym: mecps, mecdp-synthase. Engineered: yes

Source:

Burkholderia pseudomallei. Pseudomonas pseudomallei. Organism_taxid: 28450. Strain: 1710b. Gene: ispf, mecs, bpsl2098. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.05Å

R-factor:

0.207

R-free:

0.246

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

D.W.Begley et al. (2011). Leveraging structure determination with fragment screening for infectious disease drug targets: MECP synthase from Burkholderia pseudomallei. J Struct Funct Genomics, 12, 63-76. PubMed id: 21359640

Date:

24-Oct-08

Release date:

04-Nov-08

PROCHECK

	Headers

	References

Protein chain

Q3JRA0 (ISPF_BURP1) - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Burkholderia pseudomallei (strain 1710b)

Seq: Struc:					162 a.a. 158 a.a.

Protein chains

Q3JRA0 (ISPF_BURP1) - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Burkholderia pseudomallei (strain 1710b)

Seq: Struc:					162 a.a. 146 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

Chains A, B, C: E.C.4.6.1.12 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4- cyclic diphosphate + CMP

4-CDP-2-C-methyl-D-erythritol 2-phosphate	=	2-C-methyl-D-erythritol 2,4- cyclic diphosphate	+	CMP

Cofactor:

Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

	J Struct Funct Genomics 12:63-76 (2011)
PubMed id: 21359640

Leveraging structure determination with fragment screening for infectious disease drug targets: MECP synthase from Burkholderia pseudomallei.
D.W.Begley, R.C.Hartley, D.R.Davies, T.E.Edwards, J.T.Leonard, J.Abendroth, C.A.Burris, J.Bhandari, P.J.Myler, B.L.Staker, L.J.Stewart.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22868759

J.A.Bell, K.L.Ho, and R.Farid (2012).
Significant reduction in errors associated with nonbonded contacts in protein crystal structures: automated all-atom refinement with PrimeX.

Acta Crystallogr D Biol Crystallogr, 68, 935-952.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.