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PDBsum entry 3ee9
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Viral protein
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PDB id
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3ee9
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DOI no:
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Acta Crystallogr D Biol Crystallogr
65:11-17
(2009)
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PubMed id:
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Structure of NS1A effector domain from the influenza A/Udorn/72 virus.
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S.Xia,
A.F.Monzingo,
J.D.Robertus.
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ABSTRACT
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The nonstructural protein NS1A from influenza virus is a multifunctional
virulence factor and a potent inhibitor of host immunity. It has two functional
domains: an N-terminal 73-amino-acid RNA-binding domain and a C-terminal
effector domain. Here, the crystallographic structure of the NS1A effector
domain of influenza A/Udorn/72 virus is presented. Structure comparison with the
NS1 effector domain from mouse-adapted influenza A/Puerto Rico/8/34 (PR8) virus
strain reveals a similar monomer conformation but a different dimer interface.
Further analysis and evaluation shows that the dimer interface observed in the
structure of the PR8 NS1 effector domain is likely to be a crystallographic
packing effect. A hypothetical model of the intact NS1 dimer is presented.
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Selected figure(s)
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Figure 1.
Electron density for the influenza A/Udorn/72 NS1A effector
domain. This is a section of a 2F [o] [minus sign] F [c] map
contoured at 1[sigma] to show the interface of the
effector-domain dimer. Acta Crystallogr D Biol Crystallogr. 2009
January 1; 65(Pt 1): 11–17. Published online 2008 December 19.
doi: 10.1107/S0907444908032186. Copyright [copyright]
International Union of Crystallography 2009
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Figure 4.
The dimer interface for the influenza A/Udorn/72 NS1A
effector domain. (a) A space-filling model of monomer subunit A
reveals a distinct largely hydrophobic pocket that has evolved
to bind aromatic residues from the F2F3 domain of CPSF30. Here,
Trp187 from effector domain monomer subunit B (shown in blue)
binds in that pocket. (b) A detailed view of the interactions of
Trp187 with monomer subunit A binding-cleft residues (shown in
green). Acta Crystallogr D Biol Crystallogr. 2009 January 1;
65(Pt 1): 11–17. Published online 2008 December 19. doi:
10.1107/S0907444908032186. Copyright [copyright] International
Union of Crystallography 2009
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The above figures are
reprinted
from an Open Access publication published by the IUCr:
Acta Crystallogr D Biol Crystallogr
(2009,
65,
11-17)
copyright 2009.
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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B.G.Hale,
P.S.Kerry,
D.Jackson,
B.L.Precious,
A.Gray,
M.J.Killip,
R.E.Randall,
and
R.J.Russell
(2010).
Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein.
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Proc Natl Acad Sci U S A,
107,
1954-1959.
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B.G.Hale,
R.A.Albrecht,
and
A.García-Sastre
(2010).
Innate immune evasion strategies of influenza viruses.
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Future Microbiol,
5,
23-41.
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S.Xia,
and
J.D.Robertus
(2010).
X-ray structures of NS1 effector domain mutants.
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Arch Biochem Biophys,
494,
198-204.
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PDB codes:
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R.M.Krug,
and
J.M.Aramini
(2009).
Emerging antiviral targets for influenza A virus.
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Trends Pharmacol Sci,
30,
269-277.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
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so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
