PDBsum entry 3e5h

Signaling protein

PDB id

3e5h

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Contents

			Protein chain

					177 a.a. *

			Ligands

					GNP


					GOL ×2

			Metals

					_MG

			Waters ×205

* Residue conservation analysis

PDB id:

3e5h

Links
- PDBe
- RCSB
- MMDB
- JenaLib
- Proteopedia
- CATH
- SCOP
- PDBSWS
- PDBePISA
- CSA
- ProSAT

Name:

Signaling protein

Title:

Crystal structure of rab28 gtpase in the active (gppnhp-bound) form

Structure:

Ras-related protein rab-28. Chain: a. Fragment: rab28 (unp residues 11-184). Synonym: rab-26. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rab28. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.50Å

R-factor:

0.153

R-free:

0.181

Authors:

S.H.Lee,K.Baek,Y.Li,R.Dominguez

Key ref:

S.H.Lee et al. (2008). Large nucleotide-dependent conformational change in Rab28. Febs Lett, 582, 4107-4111. PubMed id: 19026641

Date:

13-Aug-08

Release date:

26-Aug-08

Supersedes:

2hy4

PROCHECK

	Headers

	References

Protein chain

P51157 (RAB28_HUMAN) - Ras-related protein Rab-28 from Homo sapiens

Seq: Struc:					221 a.a. 177 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 3 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Febs Lett 582:4107-4111 (2008)
PubMed id: 19026641

Large nucleotide-dependent conformational change in Rab28.
S.H.Lee, K.Baek, R.Dominguez.

ABSTRACT

Rab GTPases are essential regulators of membrane trafficking. We report crystal structures of Rab28 in the active (GppNHp-bound) and inactive (GDP-3'P-bound) forms at 1.5 and 1.1A resolution. Rab28 is a distant member of the Rab family. While the overall fold of Rab28 resembles that of other Rab GTPases, it undergoes a larger nucleotide-dependent conformational change than other members of this family. Added flexibility resulting from a double-glycine motif at the beginning of switch 2 might partially account for this observation. The double-glycine motif, which is conserved in the Arf family, only occurs in Rab28 and Rab7B of the Rab family, and may have a profound effect on their catalytic activities.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20167115

J.M.Escudero, J.L.Haller, C.M.Clay, and K.W.Escudero (2010).
Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter.

J Ovarian Res, 3, 4.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.