PDBsum entry 3e17

Cell adhesion

PDB id: 3e17

Contents

Protein chain

80 a.a. *

Waters ×203

* Residue conservation analysis

PDB id:

3e17

Name:

Cell adhesion

Title:

Crystal structure of the second pdz domain from human zona occludens-2

Structure:

Tight junction protein zo-2. Chain: a, b. Fragment: unp residues 306-384. Synonym: zonula occludens protein 2, zona occludens protein 2, tight junction protein 2, the second pdz domain from human zona occludens- 2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tjp2, x104, zo2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.75Å R-factor: 0.208 R-free: 0.237

Authors:

H.Chen,S.L.Tong,M.K.Teng,L.W.Niu

Key ref:

H.Chen et al. (2009). Structure of the second PDZ domain from human zonula occludens 2. Acta Crystallogr Sect F Struct Biol Cryst Commun, 65, 327-330. PubMed id: 19342771

Date:

01-Aug-08 Release date: 21-Jul-09

Protein chains

Q9UDY2  (ZO2_HUMAN) -  Tight junction protein ZO-2 from Homo sapiens

Seq: 1190 a.a.

Struc: 80 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Acta Crystallogr Sect F Struct Biol Cryst Commun 65:327-330 (2009)

PubMed id: 19342771

Structure of the second PDZ domain from human zonula occludens 2.

H.Chen, S.Tong, X.Li, J.Wu, Z.Zhu, L.Niu, M.Teng.

ABSTRACT

Human zonula occludens 2 (ZO-2) protein is a multi-domain protein that consists of an SH3 domain, a GK domain and three copies of a PDZ domain with slight divergence. The three PDZ domains act as protein-recognition modules that may mediate protein assembly and subunit localization. The crystal structure of the second PDZ domain of ZO-2 (ZO-2 PDZ2) was determined by molecular replacement at 1.75 A resolution, revealing a dimer in the asymmetric unit. The dimer is stabilized by extensive symmetrical domain-swapping of the beta1 and beta2 strands. Structural comparison shows that the ZO-2 PDZ2 homodimer may have a similar ligand-binding pattern to the ZO-1 PDZ2-connexin 43 complex.