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PDBsum entry 3ch5
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Transport protein
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PDB id
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3ch5
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Contents |
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* Residue conservation analysis
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Enzyme class 1:
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Chain A:
E.C.3.6.5.-
- ?????
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Enzyme class 2:
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Chain B:
E.C.?
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Structure
16:1116-1125
(2008)
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PubMed id:
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The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex.
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N.Schrader,
C.Koerner,
K.Koessmeier,
J.A.Bangert,
A.Wittinghofer,
R.Stoll,
I.R.Vetter.
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ABSTRACT
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Nucleoporin (Nup) 153 is a highly mobile, multifunctional, and essential nuclear
pore protein. It contains four zinc finger motifs that are thought to be crucial
for the regulation of transport-receptor/cargo interactions via their binding to
the small guanine nucleotide binding protein, Ran. We found this interaction to
be independent of the phoshorylation state of the nucleotide. Ran binds with the
highest affinity to the second zinc finger motif of Nup153 (Nup153ZnF2). Here we
present the crystal structure of this complex, revealing a new type of Ran-Ran
interaction partner interface together with the solution structure of
Nup153ZnF2. According to our complex structure, Nup153ZnF2 binding to Ran
excludes the formation of a Ran-importin-beta complex. This finding suggests a
local Nup153-mediated Ran reservoir at the nucleoplasmic distal ring of the
nuclear pore, where nucleotide exchange may take place in a ternary
Nup153-Ran-RCC1 complex, so that import complexes are efficiently terminated.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.A.DeGrasse,
K.N.DuBois,
D.Devos,
T.N.Siegel,
A.Sali,
M.C.Field,
M.P.Rout,
and
B.T.Chait
(2009).
Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor.
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Mol Cell Proteomics,
8,
2119-2130.
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J.R.Partridge,
and
T.U.Schwartz
(2009).
Crystallographic and biochemical analysis of the Ran-binding zinc finger domain.
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J Mol Biol,
391,
375-389.
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PDB codes:
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S.G.Brohawn,
J.R.Partridge,
J.R.Whittle,
and
T.U.Schwartz
(2009).
The nuclear pore complex has entered the atomic age.
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Structure,
17,
1156-1168.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
