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PDBsum entry 3c1v
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Calcium binding protein
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PDB id
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3c1v
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Calcium binding protein
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Title:
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The 1.5 a crystal structure of ca2+-bound s100a4
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Structure:
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Protein s100-a4. Chain: a, b, c, d. Synonym: s100 calcium-binding protein a4, metastasin, protein mts1, placental calcium-binding protein, calvasculin. Engineered: yes
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Source:
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Homo sapiens. Human. Gene: s100a4. Expressed in: escherichia coli.
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Resolution:
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1.50Å
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R-factor:
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0.196
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R-free:
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0.218
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Authors:
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A.R.Gingras,I.L.Barsukov
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Key ref:
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A.R.Gingras
et al.
(2008).
Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4.
Febs Lett,
582,
1651-1656.
PubMed id:
DOI:
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Date:
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24-Jan-08
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Release date:
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01-Apr-08
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PROCHECK
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Headers
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References
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P26447
(S10A4_HUMAN) -
Protein S100-A4 from Homo sapiens
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Seq:
Struc:
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101 a.a.
93 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Febs Lett
582:1651-1656
(2008)
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PubMed id:
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Crystal structure of the Ca(2+)-form and Ca(2+)-binding kinetics of metastasis-associated protein, S100A4.
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A.R.Gingras,
J.Basran,
A.Prescott,
M.Kriajevska,
C.R.Bagshaw,
I.L.Barsukov.
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ABSTRACT
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S100A4 takes part in control of tumour cell migration and contributes to
metastatic spread in in vivo models. In the active dimeric Ca(2+)-bound state it
interacts with multiple intracellular targets. Conversely, oligomeric forms of
S100A4 are linked with the extracellular function of this protein. We report the
1.5A X-ray crystal structure of Ca(2+)-bound S100A4 and use it to identify the
residues involved in target recognition and to derive a model of the oligomeric
state. We applied stopped-flow analysis of tyrosine fluorescence to derive
kinetics of S100A4 activation by Ca(2+) (k(on)=3.5 microM(-1)s(-1),
k(off)=20s(-1)).
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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V.N.Malashkevich,
N.G.Dulyaninova,
U.A.Ramagopal,
M.A.Liriano,
K.M.Varney,
D.Knight,
M.Brenowitz,
D.J.Weber,
S.C.Almo,
and
A.R.Bresnick
(2010).
Phenothiazines inhibit S100A4 function by inducing protein oligomerization.
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Proc Natl Acad Sci U S A,
107,
8605-8610.
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PDB code:
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I.Bertini,
S.Das Gupta,
X.Hu,
T.Karavelas,
C.Luchinat,
G.Parigi,
and
J.Yuan
(2009).
Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states.
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J Biol Inorg Chem,
14,
1097-1107.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
