PDBsum entry 3bfd

Hydrolase

PDB id: 3bfd

Contents

Protein chains

263 a.a. *

Ligands

CAC ×4

Waters ×1019

* Residue conservation analysis

PDB id:

3bfd

Name:

Hydrolase

Title:

Crystal structure of the class a beta-lactamase sed-g238c mutant from citrobacter sedlakii

Structure:

Class a beta-lactamase sed1. Chain: a, b, c, d. Fragment: sed-g238c, unp residues 33-295. Engineered: yes. Mutation: yes

Source:

Citrobacter sedlakii. Organism_taxid: 67826. Strain: 2596. Gene: bla-sed-1. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Resolution:

2.00Å R-factor: 0.183 R-free: 0.214

Authors:

L.Pernot,S.Petrella,W.Sougakoff

Key ref:

L.Pernot et al. Acyl-Intermediate structures of the class a beta-Lactamase sed-G238c. To be published, .

Date:

21-Nov-07 Release date: 18-Dec-07

Protein chains

Q93PQ0  (Q93PQ0_9ENTR) -  Beta-lactamase from Citrobacter sedlakii

Seq: 295 a.a.

Struc: 263 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)