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PDBsum entry 3bap
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RNA binding protein
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PDB id
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3bap
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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RNA binding protein
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Title:
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Crystal structure of the 25 kda subunit of human cleavage factor im
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Structure:
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Cleavage and polyadenylation specificity factor subunit 5. Chain: a. Synonym: cleavage and polyadenylation specificity factor 25 kda subunit, cpsf 25 kda subunit, pre-mRNA cleavage factor im 25 kda subunit, nucleoside diphosphate-linked moiety x motif 21, nudix motif 21. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: nudt21, cfim25, cpsf25, cpsf5. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.85Å
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R-factor:
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0.226
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R-free:
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0.244
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Authors:
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M.Coseno,S.Doublie
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Key ref:
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M.Coseno
et al.
(2008).
Crystal structure of the 25 kDa subunit of human cleavage factor Im.
Nucleic Acids Res,
36,
3474-3483.
PubMed id:
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Date:
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08-Nov-07
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Release date:
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19-Feb-08
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PROCHECK
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Headers
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References
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O43809
(CPSF5_HUMAN) -
Cleavage and polyadenylation specificity factor subunit 5 from Homo sapiens
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Seq:
Struc:
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227 a.a.
203 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Nucleic Acids Res
36:3474-3483
(2008)
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PubMed id:
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Crystal structure of the 25 kDa subunit of human cleavage factor Im.
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M.Coseno,
G.Martin,
C.Berger,
G.Gilmartin,
W.Keller,
S.Doublié.
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ABSTRACT
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Cleavage factor I(m) is an essential component of the pre-messenger RNA 3'-end
processing machinery in higher eukaryotes, participating in both the
polyadenylation and cleavage steps. Cleavage factor I(m) is an oligomer composed
of a small 25 kDa subunit (CF I(m)25) and a variable larger subunit of either
59, 68 or 72 kDa. The small subunit also interacts with RNA, poly(A) polymerase,
and the nuclear poly(A)-binding protein. These protein-protein interactions are
thought to be facilitated by the Nudix domain of CF I(m)25, a hydrolase motif
with a characteristic alpha/beta/alpha fold and a conserved catalytic sequence
or Nudix box. We present here the crystal structures of human CF I(m)25 in its
free and diadenosine tetraphosphate (Ap(4)A) bound forms at 1.85 and 1.80 A,
respectively. CF I(m)25 crystallizes as a dimer and presents the classical Nudix
fold. Results from crystallographic and biochemical experiments suggest that CF
I(m)25 makes use of its Nudix fold to bind but not hydrolyze ATP and Ap(4)A. The
complex and apo protein structures provide insight into the active oligomeric
state of CF I(m) and suggest a possible role of nucleotide binding in either the
polyadenylation and/or cleavage steps of pre-messenger RNA 3'-end processing.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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Q.Yang,
M.Coseno,
G.M.Gilmartin,
and
S.Doublié
(2011).
Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping.
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Structure,
19,
368-377.
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PDB codes:
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G.Martin,
A.Ostareck-Lederer,
A.Chari,
N.Neuenkirchen,
S.Dettwiler,
D.Blank,
U.Rüegsegger,
U.Fischer,
and
W.Keller
(2010).
Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I.
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RNA,
16,
1646-1659.
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Q.Yang,
G.M.Gilmartin,
and
S.Doublié
(2010).
Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing.
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Proc Natl Acad Sci U S A,
107,
10062-10067.
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PDB codes:
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S.Kim,
J.Yamamoto,
Y.Chen,
M.Aida,
T.Wada,
H.Handa,
and
Y.Yamaguchi
(2010).
Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation.
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Genes Cells,
15,
1003-1013.
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S.A.Kennedy,
M.L.Frazier,
M.Steiniger,
A.M.Mast,
W.F.Marzluff,
and
M.R.Redinbo
(2009).
Crystal structure of the HEAT domain from the Pre-mRNA processing factor Symplekin.
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J Mol Biol,
392,
115-128.
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PDB code:
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Y.Shi,
D.C.Di Giammartino,
D.Taylor,
A.Sarkeshik,
W.J.Rice,
J.R.Yates,
J.Frank,
and
J.L.Manley
(2009).
Molecular architecture of the human pre-mRNA 3' processing complex.
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Mol Cell,
33,
365-376.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
