PDBsum entry 3b9h

Transferase

PDB id: 3b9h

Contents

Protein chain

264 a.a. *

Ligands

PO4 ×2

NDU

Waters ×55

* Residue conservation analysis

PDB id:

3b9h

Name:

Transferase

Title:

E. Coli thymidylate synthase complexed with 5-nitro-2'-deoxy uridine

Structure:

Thymidylate synthase. Chain: a. Synonym: ts, tsase. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: thya. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.49Å R-factor: 0.172 R-free: 0.233

Authors:

S.A.Roberts,W.R.Montfort

Key ref:

W.B.Arendall III et al. Structural studies of early events in the thymidylate synthase reaction. To be published, .

Date:

05-Nov-07 Release date: 25-Nov-08

Protein chain

P0A884  (TYSY_ECOLI) -  Thymidylate synthase from Escherichia coli (strain K12)

Seq: 264 a.a.

Struc: 264 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.1.1.45 - thymidylate synthase.
• Pathway: Folate Coenzymes
• Reaction: dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP

dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (Bound ligand (Het Group name = NDU) matches with 86.96% similarity) = 7,8-dihydrofolate + dTMP

Molecule diagrams generated from .mol files obtained from the KEGG ftp site