 |
PDBsum entry 3azv
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Toxin
|
|
|
Title:
|
|
Crystal structure of the receptor binding domain
|
|
Structure:
|
|
D/c mosaic neurotoxin. Chain: a, b. Fragment: receptor binding domain(residues 857-1285). Synonym: neurotoxin. Engineered: yes
|
|
Source:
|
|
Clostridium botulinum. Organism_taxid: 1491. Gene: bont. Expressed in: escherichia coli. Expression_system_taxid: 562.
|
|
Resolution:
|
|
|
3.10Å
|
R-factor:
|
0.252
|
R-free:
|
0.286
|
|
|
Authors:
|
|
N.Nuemket,Y.Tanaka,K.Tsukamoto,T.Tsuji,K.Nakamura,S.Kozaki,M.Yao, I.Tanaka
|
|
Key ref:
|
|
N.Nuemket
et al.
(2011).
Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin: insight into the ganglioside binding mechanism.
Biochem Biophys Res Commun,
411,
433-439.
PubMed id:
|
|
|
Date:
|
|
|
02-Jun-11
|
Release date:
|
28-Dec-11
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q9LBR1
(Q9LBR1_CLOBO) -
Neurotoxin from Clostridium botulinum
|
|
|
|
Seq:
Struc:
|
|
|
|
1285 a.a.
418 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
E.C.3.4.24.69
- bontoxilysin.
|
|
|
|
|
|
|
Reaction:
|
|
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.
|
|
|
|
|
|
Cofactor:
|
|
Zn(2+)
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Biochem Biophys Res Commun
411:433-439
(2011)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin: insight into the ganglioside binding mechanism.
|
|
N.Nuemket,
Y.Tanaka,
K.Tsukamoto,
T.Tsuji,
K.Nakamura,
S.Kozaki,
M.Yao,
I.Tanaka.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
