PDBsum entry 3ar5

Hydrolase/hydrolase inhibitor

PDB id: 3ar5

Contents

Protein chain

995 a.a. *

Ligands

TG1

TM1

PTY ×3

Metals

_NA

Waters ×212

* Residue conservation analysis

PDB id:

3ar5

Name:

Hydrolase/hydrolase inhibitor

Title:

Calcium pump crystal structure with bound tnp-amp and tg

Structure:

Sarcoplasmic/endoplasmic reticulum calcium atpase 1. Chain: a. Synonym: serca1, sr ca(2+)-atpase 1, calcium pump 1, calcium- transporting atpase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, endoplasmic reticulum class 1/2 ca(2+) atpase. Ec: 3.6.3.8

Source:

Oryctolagus cuniculus. Rabbits. Organism_taxid: 9986. Tissue: skeletal muscle (white)

Resolution:

2.20Å R-factor: 0.262 R-free: 0.288

Authors:

C.Toyoshima,S.Yonekura,J.Tsueda,S.Iwasawa

Key ref:

C.Toyoshima et al. (2011). Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+. Proc Natl Acad Sci U S A, 108, 1833-1838. PubMed id: 21239683

Date:

24-Nov-10 Release date: 02-Feb-11

Protein chain

P04191  (AT2A1_RABIT) -  Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 from Oryctolagus cuniculus

Seq: 1001 a.a.

Struc: 994 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.7.2.2.10 - P-type Ca(2+) transporter.
• Reaction: Ca²⁺(in) + ATP + H2O = Ca²⁺(out) + ADP + phosphate + H⁺

Ca(2+)(in) + ATP + H2O = Ca(2+)(out) (Bound ligand (Het Group name = TM1) matches with 54.76% similarity) + ADP + phosphate + H(+)

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 108:1833-1838 (2011)

PubMed id: 21239683

Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+.

C.Toyoshima, S.Yonekura, J.Tsueda, S.Iwasawa.

ABSTRACT

No abstract given.