PDBsum entry 3aef

Oxidoreductase

PDB id: 3aef

Contents

Protein chains

613 a.a. *

239 a.a. *

138 a.a. *

102 a.a. *

Ligands

FAD

FES

SF4

F3S

HEM

EPH

Waters ×3

* Residue conservation analysis

PDB id:

3aef

Name:

Oxidoreductase

Title:

Crystal structure of porcine heart mitochondrial complex ii with an empty quinone-binding pocket

Structure:

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial. Chain: a. Synonym: flavoprotein subunit of complex ii, fp. Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial. Chain: b. Synonym: iron-sulfur subunit of complex ii, ip. Succinate dehydrogenase cytochrome b560 subunit,

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Organ: heart. Tissue: muscle. Tissue: muscle

Resolution:

2.80Å R-factor: 0.204 R-free: 0.251

Authors:

S.Harada,T.Sasaki,M.Shindo,Y.Kido,D.K.Inaoka,J.Omori,A.Osanai, K.Sakamoto,J.Mao,S.Matsuoka,M.Inoue,T.Honma,A.Tanaka,K.Kita

Key ref:

S.Harada et al. Crystal structure of porcine heart mitochondrial comp with an empty quinone-Binding pocket. To be published, .

Date:

04-Feb-10 Release date: 09-Feb-11

Protein chain

Q0QF01  (SDHA_PIG) -  Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial from Sus scrofa

Seq: 664 a.a.

Struc: 613 a.a.

Protein chain

Q007T0  (SDHB_PIG) -  Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial from Sus scrofa

Seq: 280 a.a.

Struc: 239 a.a.

Protein chain

D0VWV4  (C560_PIG) -  Succinate dehydrogenase cytochrome b560 subunit, mitochondrial from Sus scrofa

Seq: 169 a.a.

Struc: 138 a.a.

Protein chain

A5GZW8  (DHSD_PIG) -  Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial from Sus scrofa

Seq: 159 a.a.

Struc: 102 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.1.1.5.- - ?????
• Enzyme class 3: Chains A, B: E.C.1.3.5.1 - succinate dehydrogenase.

Pathway:

• Reaction: a quinone + succinate = fumarate + a quinol
• Cofactor: FAD; Iron-sulfur

FAD (Bound ligand (Het Group name = FAD) corresponds exactly) Iron-sulfur

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site