PDBsum entry 3a5t

Transcription regulator/DNA

PDB id: 3a5t

Contents

Protein chains

93 a.a. *

DNA/RNA

Metals

_MG

Waters ×83

* Residue conservation analysis

PDB id:

3a5t

Name:

Transcription regulator/DNA

Title:

Crystal structure of mafg-DNA complex

Structure:

Transcription factor mafg. Chain: a, b. Fragment: binding domain, residues 21-123. Synonym: mafg transcription factor, v-maf musculoaponeurotic fibrosarcoma oncogene homolog g. Engineered: yes. 5'-d( Cp Tp Gp Ap Tp Gp Ap Gp Tp Cp Ap Gp Cp Ap C)-3'. Chain: c. Engineered: yes.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: mafg. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

2.80Å R-factor: 0.261 R-free: 0.294

Authors:

H.Kurokawa,H.Motohashi,S.Sueno,M.Kimura,H.Takagawa,Y.Kanno, M.Yamamoto,T.Tanaka

Key ref:

H.Kurokawa et al. (2009). Structural basis of alternative DNA recognition by Maf transcription factors. Mol Cell Biol, 29, 6232-6244. PubMed id: 19797082

Date:

11-Aug-09 Release date: 13-Oct-09

Protein chains

O54790  (MAFG_MOUSE) -  Transcription factor MafG from Mus musculus

Seq: 162 a.a.

Struc: 93 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

C-T-G-A-T-G-A-G-T-C-A-G-C-A-C 15 bases

G-T-G-C-T-G-A-C-T-C-A-T-C-A-G 15 bases

Mol Cell Biol 29:6232-6244 (2009)

PubMed id: 19797082

Structural basis of alternative DNA recognition by Maf transcription factors.

H.Kurokawa, H.Motohashi, S.Sueno, M.Kimura, H.Takagawa, Y.Kanno, M.Yamamoto, T.Tanaka.

ABSTRACT

Maf transcription factors constitute a family of the basic region-leucine zipper (bZip) factors and recognize unusually long DNA motifs (13 or 14 bp), termed the Maf recognition element (MARE). The MARE harbors extended GC sequences on each side of its core motif, which is similar to TRE or CRE (7 or 8 bp) recognized by the AP1 and CREB/ATF families, respectively. To ascertain the structural basis governing the acquirement of such unique DNA recognition, we determined the crystal structure of the MafG-DNA complex. Each MafG monomer consists of three helices in which the carboxyl-terminal long helix organizes one DNA-contacting element and one coiled-coil dimer formation element. To our surprise, two well-conserved residues, Arg57 and Asn61 in the basic region, play critical roles in Maf-specific DNA recognition. These two residues show unique side-chain orientations and interact directly with the extended GC bases. Maf-specific residues in the amino-terminal and basic regions appear to indirectly stabilize MARE recognition through DNA backbone phosphate interactions. This study revealed an alternative DNA recognition mechanism of the bZip factors that bestows specific target gene profiles upon Maf homodimers or Maf-containing heterodimers.