PDBsum entry 3zwn

Hydrolase

PDB id: 3zwn

Contents

Protein chains

253 a.a.

Ligands

CGR

NGD

Waters ×452

PDB id:

3zwn

Name:

Hydrolase

Title:

Crystal structure of aplysia cyclase complexed with substrate ngd and product cgdpr

Structure:

Adp-ribosyl cyclase. Chain: a, b. Synonym: adrc, NAD glycohydrolase, NAD(+) nucleosidase, nadase. Engineered: yes

Source:

Aplysia californica. California sea hare. Organism_taxid: 6500. Expressed in: pichia pastoris. Expression_system_taxid: 4922.

Resolution:

1.80Å R-factor: 0.179 R-free: 0.233

Authors:

M.Kotaka,R.Graeff,L.H.Zhang,H.C.Lee,Q.Hao

Key ref:

M.Kotaka et al. (2012). Structural studies of intermediates along the cyclization pathway of Aplysia ADP-ribosyl cyclase. J Mol Biol, 415, 514-526. PubMed id: 22138343

Date:

02-Aug-11 Release date: 30-Nov-11

Protein chains

P29241  (NADA_APLCA) -  ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase from Aplysia californica

Seq: 282 a.a.

Struc: 253 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.2.4.99.20 - 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase.
• Reaction: nicotinate + NADP⁺ = nicotinate-adenine dinucleotide phosphate + nicotinamide

nicotinate (Bound ligand (Het Group name = NGD) matches with 86.00% similarity) + NADP(+) = nicotinate-adenine dinucleotide phosphate + nicotinamide

• Enzyme class 2: E.C.3.2.2.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Mol Biol 415:514-526 (2012)

PubMed id: 22138343

Structural studies of intermediates along the cyclization pathway of Aplysia ADP-ribosyl cyclase.

M.Kotaka, R.Graeff, Z.Chen, L.H.Zhang, H.C.Lee, Q.Hao.

ABSTRACT

No abstract given.