PDBsum entry 3zn6

Viral protein

PDB id: 3zn6

Contents

Protein chains

221 a.a.

145 a.a.

Metals

_NA

_CL

Waters ×395

PDB id:

3zn6

Name:

Viral protein

Title:

Vp16-vp17 complex, a complex of the two major capsid proteins of bacteriophage p23-77

Structure:

Vp17. Chain: a. Engineered: yes. Vp16. Chain: b. Engineered: yes. Other_details: subunit of a strand-swapped homodimer

Source:

Thermus phage p23-77. Organism_taxid: 668994. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.53Å R-factor: 0.173 R-free: 0.197

Authors:

I.Rissanen,J.M.Grimes,A.Pawlowski,S.Mantynen,K.Harlos,J.K.H.Bamford, D.I.Stuart

Key ref:

I.Rissanen et al. (2013). Bacteriophage P23-77 capsid protein structures reveal the archetype of an ancient branch from a major virus lineage. Structure, 21, 718-726. PubMed id: 23623731 DOI: 10.1016/j.str.2013.02.026

Date:

13-Feb-13 Release date: 15-May-13

Protein chain

C8CHL5  (C8CHL5_9VIRU) -  VP17 from Thermus virus P23-77

Seq: 291 a.a.

Struc: 221 a.a.

Protein chain

C8CHL4  (C8CHL4_9VIRU) -  VP16 from Thermus virus P23-77

Seq: 173 a.a.

Struc: 145 a.a.

DOI no: 10.1016/j.str.2013.02.026

Structure 21:718-726 (2013)

PubMed id: 23623731

Bacteriophage P23-77 capsid protein structures reveal the archetype of an ancient branch from a major virus lineage.

I.Rissanen, J.M.Grimes, A.Pawlowski, S.Mäntynen, K.Harlos, J.K.Bamford, D.I.Stuart.

ABSTRACT

It has proved difficult to classify viruses unless they are closely related since their rapid evolution hinders detection of remote evolutionary relationships in their genetic sequences. However, structure varies more slowly than sequence, allowing deeper evolutionary relationships to be detected. Bacteriophage P23-77 is an example of a newly identified viral lineage, with members inhabiting extreme environments. We have solved multiple crystal structures of the major capsid proteins VP16 and VP17 of bacteriophage P23-77. They fit the 14 Å resolution cryo-electron microscopy reconstruction of the entire virus exquisitely well, allowing us to propose a model for both the capsid architecture and viral assembly, quite different from previously published models. The structures of the capsid proteins and their mode of association to form the viral capsid suggest that the P23-77-like and adeno-PRD1 lineages of viruses share an extremely ancient common ancestor.