PDBsum entry 3vvc

Lyase

PDB id: 3vvc

Contents

Protein chain

318 a.a.

Ligands

NAP

SO4 ×5

Waters ×82

PDB id:

3vvc

Name:

Lyase

Title:

Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form

Structure:

Capsular polysaccharide synthesis enzyme cap8e. Chain: a. Fragment: 4.2.1.115. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 158878. Strain: mu50. Gene: cape. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.196 R-free: 0.265

Authors:

T.Miyafusa,J.M.Caaveiro,Y.Tanaka,K.Tsumoto

Key ref:

T.Miyafusa et al. Crystal structure of the capsular polysaccharide synthesizing protein cape of staphylococcus aureus.. Biosci rep, . PubMed id: 23611437

Date:

18-Jul-12 Release date: 12-Jun-13

Protein chain

A0A0H3JPH0  (A0A0H3JPH0_STAAM) -  UDP-glucose 4-epimerase from Staphylococcus aureus (strain Mu50 / ATCC 700699)

Seq: 342 a.a.

Struc: 318 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.5.1.3.2 - UDP-glucose 4-epimerase.
• Pathway: UDP-glucose, UDP-galactose and UDP-glucuronate Biosynthesis
• Reaction: UDP-alpha-D-glucose = UDP-alpha-D-galactose
• Cofactor: NAD(+)

NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site