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PDBsum entry 3vml
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Oxidoreductase
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PDB id
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3vml
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PDB id:
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Oxidoreductase
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Title:
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Chimera 3-isopropylmalate dehydrogenase between shewanella oneidensis mr-1 (o) and shewanella benthica db21 mt-2 (m) from n-terminal: 20% o middle 70% m residual 10% o
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Structure:
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3-isopropylmalate dehydrogenase. Chain: a. Synonym: 3-ipm-dh, beta-ipm dehydrogenase, imdh. Engineered: yes. Other_details: chimera protein of 3-isopropylmalate dehydrogenase from shewanella oneidensis mr-1 (unp q8e9n3 residues 2-68), 3- isopropylmalate dehydrogenase from shewanella benthica db21 mt-2 (unp d2yzl2 residues 69-328), and 3-isopropylmalate dehydrogenase from shewanella oneidensis mr-1 (unp q8e9n3 residues 329-364)
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Source:
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Shewanella oneidensis, shewanella benthica. Organism_taxid: 211586, 43661. Strain: mr-1, db21 mt-2, mr-1. Gene: leub, so_4235. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.56Å
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R-factor:
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0.179
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R-free:
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0.209
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Authors:
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T.Nagae,N.Watanabe
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Key ref:
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Y.Hamajima
et al.
Structure analysis of chimeric 3-Isopropylmalate dehydrogenase between the obligate piezophile shewane benthica db21mt-2 and the nonpiezophile shewanella oneidensis mr-1.
To be published,
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Date:
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13-Dec-11
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Release date:
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26-Dec-12
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PROCHECK
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Headers
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References
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Enzyme class:
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E.C.1.1.1.85
- 3-isopropylmalate dehydrogenase.
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Pathway:
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Leucine Biosynthesis
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Reaction:
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(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
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(2R,3S)-3-isopropylmalate
Bound ligand (Het Group name = )
corresponds exactly
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+
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NAD(+)
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=
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4-methyl-2-oxopentanoate
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+
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CO2
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+
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NADH
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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}
}
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