spacer
spacer

PDBsum entry 3vml

Go to PDB code: 
protein ligands metals links
Oxidoreductase PDB id
3vml

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chain
363 a.a.
Ligands
IPM
Metals
_CL
_MG
Waters ×472
PDB id:
3vml
Name: Oxidoreductase
Title: Chimera 3-isopropylmalate dehydrogenase between shewanella oneidensis mr-1 (o) and shewanella benthica db21 mt-2 (m) from n-terminal: 20% o middle 70% m residual 10% o
Structure: 3-isopropylmalate dehydrogenase. Chain: a. Synonym: 3-ipm-dh, beta-ipm dehydrogenase, imdh. Engineered: yes. Other_details: chimera protein of 3-isopropylmalate dehydrogenase from shewanella oneidensis mr-1 (unp q8e9n3 residues 2-68), 3- isopropylmalate dehydrogenase from shewanella benthica db21 mt-2 (unp d2yzl2 residues 69-328), and 3-isopropylmalate dehydrogenase from shewanella oneidensis mr-1 (unp q8e9n3 residues 329-364)
Source: Shewanella oneidensis, shewanella benthica. Organism_taxid: 211586, 43661. Strain: mr-1, db21 mt-2, mr-1. Gene: leub, so_4235. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.56Å     R-factor:   0.179     R-free:   0.209
Authors: T.Nagae,N.Watanabe
Key ref: Y.Hamajima et al. Structure analysis of chimeric 3-Isopropylmalate dehydrogenase between the obligate piezophile shewane benthica db21mt-2 and the nonpiezophile shewanella oneidensis mr-1. To be published, .
Date:
13-Dec-11     Release date:   26-Dec-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
D2YZL2  (D2YZL2_9GAMM) -  3-isopropylmalate dehydrogenase from Shewanella benthica
Seq:
Struc:
364 a.a.
363 a.a.*
Protein chain
Pfam   ArchSchema ?
Q8E9N3  (LEU3_SHEON) -  3-isopropylmalate dehydrogenase from Shewanella oneidensis (strain MR-1)
Seq:
Struc:
364 a.a.
363 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 56 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.1.1.85  - 3-isopropylmalate dehydrogenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Leucine Biosynthesis
      Reaction: (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
(2R,3S)-3-isopropylmalate
Bound ligand (Het Group name = IPM)
corresponds exactly
+ NAD(+)
= 4-methyl-2-oxopentanoate
+ CO2
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

spacer

spacer