PDBsum entry 3v7d

Cell cycle

PDB id

3v7d

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Contents

			Protein chains

					139 a.a.


					450 a.a.


					139 a.a.

			Ligands

					PHE-ASN-GLY-LEU- THR-SEP-PRO-GLN- ARG-SEP

			Waters ×320

PDB id:

3v7d

Links

Name:

Cell cycle

Title:

Crystal structure of scskp1-sccdc4-psic1 peptide complex

Structure:

Suppressor of kinetochore protein 1. Chain: a, c. Synonym: centromere DNA-binding protein complex cbf3 subunit d, e3 ubiquitin ligase complex scf subunit skp1. Engineered: yes. Cell division control protein 4. Chain: b, d. Fragment: unp residues 263-744. Synonym: e3 ubiquitin ligase complex scf subunit cdc4, f-box protein

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: cbf3d, d9798.14, skp1, ydr328c. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cdc4, yfl009w. Synthetic: yes.

Resolution:

2.31Å

R-factor:

0.197

R-free:

0.221

Authors:

X.Tang,S.Orlicky,T.Mittag,V.Csizmok,T.Pawson,J.Forman-Kay,F.Sicheri, M.Tyers

Key ref:

X.Tang et al. (2012). Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase. Proc Natl Acad Sci U S A, 109, 3287-3292. PubMed id: 22328159

Date:

20-Dec-11

Release date:

02-May-12

PROCHECK

	Headers

	References

Protein chain

P52286 (SKP1_YEAST) - Suppressor of kinetochore protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					194 a.a. 139 a.a.

Protein chains

P07834 (CDC4_YEAST) - Cell division control protein 4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:					779 a.a. 450 a.a.*

Protein chain

P52286 (SKP1_YEAST) - Suppressor of kinetochore protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					194 a.a. 139 a.a.

Key:

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

	Proc Natl Acad Sci U S A 109:3287-3292 (2012)
PubMed id: 22328159

Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase.
X.Tang, S.Orlicky, T.Mittag, V.Csizmok, T.Pawson, J.D.Forman-Kay, F.Sicheri, M.Tyers.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

23314252

N.A.Lyons, B.R.Fonslow, J.K.Diedrich, J.R.Yates, and D.O.Morgan (2013).
Sequential primed kinases create a damage-responsive phosphodegron on Eco1.

Nat Struct Mol Biol, 20, 194-201.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.